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5,5'-Dithiobis(2-nitrobenzoic acid) (Ellmans reagent, DTNB)
Supplier:
Thermo Scientific Chemicals
Description:
Reagent for determination of sufhydryl groups
Catalog Number:
(TS11754-0250)
Supplier:
THERMO FISHER SCIENTIFIC CHEMICALS
Description:
5,5'-Dithiobis(2-nitrobenzoic acid) (Ellmans reagent, DTNB) 99%
Catalog Number:
(80056-346)
Supplier:
MilliporeSigma
Description:
5,5''-Dithio-bis-(2-nitrobenzoic Acid). (DTNB; Ellman'' s reagent). Pale yellow solid. PROTECT FROM LIGHT. PROTECT FROM MOISTURE. Sulfhydryl reagent used to characterize reacting SH groups. Useful in photometric determination of thiols.
Supplier:
Enzo Life Sciences
Description:
Sensitive sulfhydyl reagent
Catalog Number:
(82022-846)
Supplier:
G-Biosciences
Description:
Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow colored product at 412nm.
Ellman's reagent is very useful as a free sulfhydryl assay reagent due to its high specificity for -SH groups at neutral pH, high molar extinction coefficient and short reaction time.
Supplier:
BeanTown Chemical
Description:
CAS: 69-78-3; EC No: 200-714-4; MDL No: MFCD00007140; RTECS: DG9650000
Powder; Molecular Formula: C14H8N2O8S2; MW: 396.35
Melting Point: 240-245° (decomposes)
Supplier:
AMBEED, INC
Description:
5,5'-Dithiobis(2-nitrobenzoic acid) (Ellmans reagent, DTNB) 98%
Catalog Number:
(PI22582)
Supplier:
Invitrogen
Description:
Thermo Scientific Pierce Ellman's Reagent (DTNB) reacts with sulfhydryl groups to yield a colored product, providing a reliable method to measure reduced cysteines and other free sulfhydryls in solution.
Supplier:
Adipogen
Description:
A sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues. Used to characterize reactive thiol groups and photometric determination of thiols and for measuring low-molecular mass thiols such as glutathione in both pure solutions and biological samples, such as blood. It can also measure the number of thiol groups on proteins. Through reaction with aliphatic thiol groups a mixed disulfide of protein thiol and one mole of 2-nitro-5-thiobenzoate per mole of protein sulfhydryl group is being formed. DTNB has little absorbance. Reaction with -SH groups on proteins (from any solvent accessible Cys) under mild alkaline conditions (pH 7-8) produces the 2-nitro-5-thiobenzoate anion, which gives an intense yellow color with an absorption maximum at 409.5nm (Extinction coefficient: 14150 M-1*cm-1). Sensitive to various buffer ions, therefore, the extinction coefficient used to calculate the number of sulfhydryl groups must be matched to the reaction conditions. In case the thiol groups are in disulfide bonds, they must be reduced under anaerobic conditions prior to reaction with DTNB.
Catalog Number:
(102843-490)
Supplier:
Matrix Scientific
Description:
5,5'-Dithiobis(2-nitrobenzoic acid) (Ellmans reagent, DTNB) ≥97%
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 is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the
is still displayed and you need assistance, please call us at 1-800-932-5000.
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