Vectors, Plasmids and Libraries
Supplier:
GE Healthcare - Life Sciences
Description:
Poly(A) (polyadenylic acid) can be used to evaluate the binding of potential therapeutic agents to mRNA in drug development research.
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Supplier:
Agilent Technologies
Description:
The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. This system works with a broad range of hosts and infects both dividing and non-dividing cells.
Supplier:
Agilent Technologies
Description:
The InterPlay Mammalian TAP system allows you to recover interacting proteins from mammalian cells.
Supplier:
Agilent Technologies
Description:
The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.
Supplier:
Agilent Technologies
Description:
The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.
Catalog Number:
(95017-592)
Supplier:
GE Healthcare - Life Sciences
Description:
The <i>E. coli </i>BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.
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Supplier:
Agilent Technologies
Description:
Combine standard DNA components to build custom vector constructs from Agilent’s set of standard parts.
Supplier:
Agilent Technologies
Description:
The InterPlay Adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System, for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.
Catalog Number:
(IC821226)
Supplier:
MP Biomedicals
Description:
pUC 18 is a small vector of approximately 2.7 Kb containing the Pvu II/EcoR I fragment of pBR322 and the lacZ gene multiple cloning site from M13mp18.
Supplier:
GE Healthcare - Life Sciences
Description:
The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.
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Supplier:
Agilent Technologies
Description:
The AAV helper-free system eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer.
Catalog Number:
(89157-406)
Supplier:
Enzo Life Sciences
Description:
pJY2 encodes tRNAUCUArg to suppress lysine misincorporation at AGA codons and T7 lysozyme to depress constitutive expression of the cloned gene. pJY2 can also be used as a source of tRNAUCUArg with expression systems, such as the pGEX series of vectors, that do not rely on T7 polymerase.
Supplier:
Promega Corporation
Description:
These untagged vectors are used for inducible expression of proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Supplied as vectors used with the Flexi Cloning System.
Catalog Number:
(PAG7971)
Supplier:
Promega Corporation
Description:
These multiple cloning site vectors are used for inducible expression of HaloTag-polyhistidine fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.
Catalog Number:
(PAG8261)
Supplier:
Promega Corporation
Description:
These vectors are used for inducible expression of polyhistidine-HaloTag fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.
Catalog Number:
(PAL1081)
Supplier:
Promega Corporation
Description:
Designed for use with Insect Cell Extracts and contain untranslated region (UTR) sequences at the 5´ and 3´ ends of the gene coding region to enhance translation efficiency.
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