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Chemiluminescent Westerns are popular assays for assessing protein expression. In this indirect detection method chemiluminescent substrates emit light when reacted with an antibody conjugated to an enzyme. The emitted light is captured and archiv...
Detecting in-gel fluorescence enables the analysis of protein gels immediately after electrophoresis without staining or Western blotting. Separation of proteins by electrophoresis is a cornerstone of protein analysis. After separation identificat...
Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...
Normalization is critical for obtaining accurate quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel and for differences in transfer efficiency across a blot. Traditionally protein ...
MEDIA AND BUFFER PREPARATION SERVICES ACCELERATE SCIENTIFIC RESEARCH
Problem During a Lean Six Sigma business process assessment at one of the world’s largest pharmaceutical companies, VWRCATALYST found that scientists were spending substantial ti...
Chemiluminescent Westerns Chemiluminescent Western blotting is an indirect method for detecting proteins bound on a membrane. The method relies on an enzyme-substrate reaction that emits light which is traditionally detected on x-ray film. Chemilu...
Consistent excellence for bioanalysis Thermo Scientific SOLAµ SPE Plates Technical Guide Figure 1: SOLAµ SPE design – limiting issues associated with conventional SPE formats What is required of the bioanalytical method to meet these demands? • Ro...
Fluorescent Western blots are the gold standard for quantitative Westerns. They are ideal for detecting multiple proteins simultaneously (multi-plexing) allowing in-lane normalization and detection of your protein of interest and loading control a...
The advent of digital imaging has transformed Western blot detection. Here we outline how Western blotting has changed and review qualities to look for in a digital imager. Chemiluminescence and fluorescence When most people think of imaging their...
Cell culture ☐ Animal and Human Sera Fetal Bovine Serum (FBS) FBS Alternatives Bovine Serum Albumin (BSA) Other Sera ☐ Classical Media DMEM IMDM F-12 Leibovitz’s L-15 MCDB 131 MEM RPMI ☐ Serum-Free and Specialty Media CHO Insect Serum-Free (Genera...