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What is western blotting? Overview of western blotting Western blotting is the technique for identifying proteins of interest in a sample using gel electrophoresis to separate sample proteins. This technique has paved the way in biomedical science...

Introduction to SDS-PAGE – Separating Proteins Based on Molecular Weight Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis or SDS-PAGE is a widely used technique for separating proteins based on their molecular weight. In this technique pr...

Detecting in-gel fluorescence enables the analysis of protein gels immediately after electrophoresis without staining or Western blotting. Separation of proteins by electrophoresis is a cornerstone of protein analysis. After separation identificat...

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Protein Sample Preparation for Mass Spectrometry Protein sample preparation In order to run a successful mass spectrometry analysis you will need to properly prepare your sample. This includes a number of steps that may be specialized depending on...

Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...