flashBAC™ Baculovirus Expression Systems, Mirus Bio
Supplier: MIRUS BIO LLC.
The flashBAC system is a streamlined platform designed for the production of recombinant baculoviruses.
- Ease-of-use - single-step baculovirus generation in insect cells, no plaque purification required
- Compatibility - ready to use with most transfer vectors (PDF), including BacMam
- More protein - high recombinant protein yields
Further nonessential gene deletions in flashBAC ULTRA provide enhanced quality and yield for difficult to express proteins. The insertion of a bacterial artificial chromosome (BAC) at the AcMNPV polyhedrin gene locus allows viral DNA to be maintained and propagated as a circular genome within bacterial cells. The genomic DNA can then be purified and is the flashBAC DNA provided with this kit. To generate recombinant baculovirus using the flashBAC system, insect cells are transfected with TransIT® -Insect Transfection Reagent, flashBAC DNA a