Difco™ PPLO Media (Mycoplasma Media), BD
Supplier: Seca Med.Waagen u.Meßsysteme
PPLO (Mycoplasma) agars and broths, when supplemented with nutritive enrichments, are used for isolating and cultivating Mycoplasma.
- Meat digests, peptones, beef extract, and yeast extract provide nutrients
- Sodium chloride maintains osmotic equilibrium
Agar, the solidifying agent, is used in PPLO (Mycoplasma) Agar at a concentration slightly reduced from usual to ensure formation of the largest possible colonies because the organisms grow into the agar with only slight surface growth.
Mycoplasma Supplement contains fresh yeast extract and horse serum. Yeast extract provides the preformed nucleic acid precursors that are required by Mycoplasma species. Horse serum supplies cholesterol, a growth stimulant.
Mycoplasma Enrichment without Penicillin is a selective enrichment containing the inhibitor thallium acetate, to which a penicillin of choice can be added at the time of use to make it selective against gram-positive and gram-negative bacteria.
Mycoplasma Broth Base (Frey) is used for the cultivation of avian mycoplasmas.
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