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AMV Reverse Transcriptase

Supplier: EBRO Electronic GmbH

AMV Reverse Transcriptase

AMV Reverse Transcriptase

AMV Reverse Transcriptase

AMV Reverse Transcriptase

M5101 M5108

PAM5101EA 254.04 USD

PAM5101 PAM5108

AMV Reverse Transcriptase

Enzymes

AMV RT catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids.

For sequencing, mutagenesis, cDNA synthesis, cDNA cloning and primer extension
Efficient Reverse Transcription of Templates with High Secondary Structure
DNA polymerization using template DNA, RNA or RNA:DNA hybrids
Requires a primer as well as magnesium or manganese
Stable at higher reaction temperatures (37-58°C)
Supplied at a concentration of 10u/microl (Cat.# M5101, M5108) or 20-25u/µl (Cat.# M9004)

Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT) catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids. It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg(2+) or Mn(2+). The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.

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