RealTime-Glo MT Cell Viability Assay, Promega
Supplier: EBRO Electronic GmbH
The RealTime-Glo MT Cell Viability Assay is a non-lytic, homogeneous, bioluminescent method to determine in real time the number of viable cells in culture by measuring the reducing potential of cells and thus metabolism (MT).
- Monitor Cell Viability in Real Time
- Save time, cell culture and reagent costs
- Rapidly detect changes in cell viability
The RealTime-Glo MT Cell Viability Assay is a non-lytic, homogeneous, bioluminescent method to determine in real time the number of viable cells in culture by measuring the reducing potential of cells and thus metabolism (MT). The assay involves adding NanoLuc luciferase and a cell-permeant pro-NanoLuc substrate to cells in culture. Viable cells reduce the proprietary pro-substrate to generate a substrate for NanoLuc luciferase. This substrate diffuses from cells into the surrounding culture medium, where it is rapidly used by the NanoLuc enzyme to produce a luminescent signal. The signal correlates with the number of viable cells, making the assay well suited for cytotoxicity studies. The reagent is stable and nontoxic to cells for up to 72 hours. No cell washing, removal of medium or further reagent addition is required to determine the number of viable cells. The non-lytic nature of this assay enables cells to be monitored over time in the same well, which reduces the amount of cells used and cell culture costs, and in downstream applications, including assay multiplexing and nucleic acid analysis.
For the continuous-read protocol, where reagents are added directly to the cell culture or test compound dilution, RealTime-Glo MT Cell Viability, 100 Reactions contains sufficient reagents for 100 reactions containing 50 μL of 2X RealTime-Glo reagent per well of a 96-well plate or 400 reactions containing 12.5 μL of 2X RealTime-Glo reagent per well of a 384-well plate. Likewise, RealTime-Glo MT Cell Viability, 10 x 100 Reactions contains sufficient reagents for 1000 reactions containing 50 μL of 2X RealTime-Glo reagent per well of a 96-well plate or 4000 reactions containing 12.5 μL of 2X RealTime-Glo Reagent per well of a 384-well plate, and RealTime-Glo MT Cell Viability, 1000 Reactions contains sufficient reagents for 1000 reactions containing 50 μL of 2X RealTime-Glo reagent per well of a 96-well plate or 4000 reactions containing 12.5 μL of 2X RealTime-Glo reagent per well of a 384-well plate.
Viable cells reduce the proprietary pro-substrate to generate a substrate for NanoLuc luciferase. This substrate diffuses from cells into the surrounding culture medium where it is rapidly used by the NanoLuc enzyme to produce a luminescent signal. The signal correlates with the number of viable cells, making the assay well suited for cytotoxicity studies. The reagent is stable and nontoxic to cells for up to 72 hours. No cell washing, removal of medium, or further reagent addition is required to determine the number of viable cells. The non-lytic nature of this assay enables cells to be monitored over time in the same well, which reduces the amount of cells used and cell culture costs, and in downstream applications, including assay multiplexing and nucleic acid analysis.
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...