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Citrate Buffer (10X), pH 6.0, Electron Microscopy Sciences

Supplier: EPREDIA

Citrate buffer chemical structure

Citrate buffer chemical structure

64142-07 64142-08

103303-062EA 124.98 USD

103303-062 103305-698

Citrate Buffer (10X), pH 6.0, Electron Microscopy Sciences

Buffers Biological Buffers

This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

1X buffer solution intended for heat-induced antigen retriever in IHC
Use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens
Please refer to primary antibody protocol
Boiling point: 100 °C
Density: 0.9989 g/cm³ (8.336 lbs/gal.)

In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC.

Preparation of working solution: dilute this 10X buffer as needed (e.g. 90 ml of deionized or distilled water+10 ml of this buffer) mix well, 1X buffer can be stored at 2 to 8 °C.

The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.

Precaución: Do not freeze.

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