64142-11
64142-12
103303-056EA
124.98
USD
InStock
103303-056
103305-626
Tris Buffer (10X), pH 10.0, Electron Microscopy Sciences
Buffers
Biological Buffers
This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
- 1X buffer solution intended for heat-induced antigen retriever in IHC
- Please refer to primary antibody protocol
In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC.
Preparation of Working Solution: Dilute this 10X buffer as needed (e.g. 90 mL of deionized or distilled water+10 ml of this buffer) mix well, 1X buffer can be stored at 2 to 8 °C.
The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Precaución: Store at 2 - 8 °C. Do not freeze.