Nano-Glo® Live Cell Assay System, Promega

Supplier: EBRO Electronic GmbH

N2011 N2012 N2013
76201-570EA 252.53 USD
76201-570 76201-572 76201-574
Nano-Glo® Live Cell Assay System, Promega
Assays Cellular Assays

The Nano-Glo® Live Cell Assay System is a single-addition, non-lytic detection reagent used to measure NanoBiT® or NanoLuc® luminescence from living cells.


  • Substrate and buffer optimized to enhance stability
  • Use to detect NanoBiT® protein complementation or NanoLuc® reporter activity
  • Monitors luminescence at a single time point or continuously for up to 2 hours without compromising cell viability


The reagent is prepared by diluting the Nano-Glo® Live Cell Substrate with the Nano-Glo® LCS Dilution Buffer to make the Nano-Glo® Live Cell Reagent, a 5X stock that is added directly to cell culture medium. Both substrate and buffer solutions are optimized to provide enhanced stability.


The Nano-Glo® Live Cell Reagent is designed to reduce autoluminescence in the presence or absence of serum, increasing the sensitivity for detection of low levels of NanoBiT® or NanoLuc® luminescence. The Nano-Glo® Live Cell Assay System can be used to monitor luminescence at a user-defined time point or continuously for up to 2 hours without compromising cell viability.


The signal stability observed after addition of the Nano-Glo® Live Cell Reagent to culture media can be influenced by experimental parameters such as high levels of enzyme activity, temperature or the presence of FBS in the media.

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