Nano-Glo® Live Cell Assay System, Promega
Supplier: EBRO Electronic GmbH
The Nano-Glo® Live Cell Assay System is a single-addition, non-lytic detection reagent used to measure NanoBiT® or NanoLuc® luminescence from living cells.
- Substrate and buffer optimized to enhance stability
- Use to detect NanoBiT® protein complementation or NanoLuc® reporter activity
- Monitors luminescence at a single time point or continuously for up to 2 hours without compromising cell viability
The reagent is prepared by diluting the Nano-Glo® Live Cell Substrate with the Nano-Glo® LCS Dilution Buffer to make the Nano-Glo® Live Cell Reagent, a 5X stock that is added directly to cell culture medium. Both substrate and buffer solutions are optimized to provide enhanced stability.
The Nano-Glo® Live Cell Reagent is designed to reduce autoluminescence in the presence or absence of serum, increasing the sensitivity for detection of low levels of NanoBiT® or NanoLuc® luminescence. The Nano-Glo® Live Cell Assay System can be used to monitor luminescence at a user-defined time point or continuously for up to 2 hours without compromising cell viability.
The signal stability observed after addition of the Nano-Glo® Live Cell Reagent to culture media can be influenced by experimental parameters such as high levels of enzyme activity, temperature or the presence of FBS in the media.
Learn more

About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...