NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 5), New England Biolabs
Supplier: W.u.G. Dickgießer GmbH
Designed for use in library prep for DNA, ChIP DNA and RNA (but not Small RNA), the NEBNext Adaptors enable high-efficiency adaptor ligation and high library yields, with minimized adaptor-dimer formation.
- Highly efficient adaptor ligation
- Minimized adaptor-dimer formation
- Includes index primers for library multiplexing
- Combine with sets 1, 3, and 4 for 384 barcodes
Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER Enzyme (a combination of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing. This kit includes 96 pre-mixed unique pairs of i5 and i7 index primers, packaged in a single-use