You Searched For: Charles River Laboratories

Supplier: Corning

Description: Ham's F-12 media was originally formulated for the serum-free culture of CHO cells, and is based on Ham's F-10 medium with increased concentrations of choline, inositol, putrescine, and several amino acids

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Supplier: Corning

Description: This product is a mix of the antibiotics Penicillin (10,000 IU/mL) and Streptomycin (10,000 μg/ml) in a 100-fold working concentration. Penicillin (Penicillin G) works by inhibiting peptidoglycan synthesis, while Streptomycin inhibits protein synthesis. Penicillin-Streptomycin is effective against Gram-negative and Gram-positive bacteria.

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Supplier: Corning

Description: Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7.2 - 7.4 in a culture environment

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Supplier: Corning

Description: McCoy's 5A medium was originally formulated to meet the culture requirements of Novikoff Hepatoma cells, and has been found to support the indefinite proliferation of Walker256 carcinoma cells along with a wide range of human and rat normal and transformed cells.  Originally modified by Iwakata and Grace, this version of McCoy's 5A includes increased levels of inositol and glucose

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Catalog Number: (45000-668)

Supplier: Corning

Description: Cellstripper® is a non-enzymatic cell dissociation solution formulated with a proprietary mixture of chelators that gently dislodge adherent cells in tissue culture. It is an alternative to trypsin when used with either serum-free or serum-supplemented media. Because Cellstripper® is a gentler reagent than trypsin, cells can be exposed to Cellstripper for longer periods of time. Cellstripper® achieves superior results in comparison to enzymatic dissociation products.

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Catalog Number: (45000-616)

Supplier: Corning

Description: This is a combination of penicillin, streptomycin and Amphotericin formulated to contain 10000 units/ml penicillin G, 10 mg/ml streptomycin sulfate and 25 µg/ml amphotericin B.

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Catalog Number: (45000-690)

Supplier: Corning

Description: HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2

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Supplier: Corning

Description: Molecular biology grade water is ideal for a wide variety of routine tasks requiring a high degree of sterility Molecular biology grade water is ideal for the preparation of reagents, rinsing glassware and plasticware, and other molecular biology applications where RNase-, DNase-, and Protease-free water is required. No toxic agents, including DEPC, are used in the preparation of Mediatech's molecular biology grade water, eliminating possible interferences with enzymatic reactions.

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Supplier: Corning

Description: 0,05% Trypsin/0,53 mM EDTA in HBSS (Hank’s Balanced Salt Solution) with sodium bicarbonate, without calcium and magnesium.

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Catalog Number: (45000-700)

Supplier: Corning

Description: The addition of MEM nonessential amino acids to medium can improve growth and viability of propagating cells in culture while reducing the biosynthetic burden on these cells

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Catalog Number: (45001-122)

Supplier: Corning

Description: EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+.  These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity.  The use of EDTA therefore allows for greater control over many experiments.

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Catalog Number: (45000-676)

Supplier: Corning

Description: L-glutamine is an essential amino acid and a key component of culture media, serving as a major energy source for propagating cells. It is very stable as a dry powder and as a frozen solution but degrades rapidly in liquid media or stock solutions, producing toxic compounds. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use. This formulation is prepared in cell culture grade water.

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Catalog Number: (45000-354)

Supplier: Corning

Description: A modification of Ham's F-12, F-12K contains higher concentrations of amino acids, sodium pyruvate and other components than the original

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Supplier: Corning

Description: Named for the site at which it was developed, the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy's 5A containing an increased level of inositol.

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Catalog Number: (45001-050)

Supplier: Corning

Description: Terrific Broth is a medium for the propagation and maintenance of recombinant E.coli.

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Supplier: Corning

Description: Tris-HCl buffer has many applications in molecular biology work, including use in nucleic acid purification

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