N3013L
N3013S
101228-438EA
326
USD
InStock
101228-438
101228-440
Unmethylated Lambda DNA, New England Biolabs
Vectors, Plasmids and Libraries
Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7)
Therefore, those deoxyadenosine positions which are methylated in a wild type E. coli host are not methylated in the dam- host. This DNA gives complete digests with restriction endonucleases that are sensitive to dam methylation. Lambda DNA (N6-methyladenine-free) is 48,502 base pairs in length. The phage is isolated from a heat-inducible E. coli (dam-) lambda lysogen. The DNA is isolated from purified phage by phenol extraction and dialyzed against 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.
The bacteriophage is grown in an E. coli host which is deficient in adenine methylase (dam-).