2-Mercaptoethanol
Supplier:
VWR
Description:
2-Mercaptoethanol 99, Proteomics grade Electrophoresis, Molecular_Biology
Catalog Number:
(TCM0058-25G)
Supplier:
TCI America
Description:
CAS Number: 60-24-2
MDL Number: MFCD00004890 Molecular Formula: C2H6OS Molecular Weight: 78.13 Purity/Analysis Method: >98.0% (GC) Form: Clear Liquid Boiling point (°C): 158 Melting point (°C): -100 Flash Point (°C): 74 Specific Gravity (20/20): 1.12
Supplier:
TCI America
Description:
CAS Number: 60-24-2
MDL Number: MFCD00004890 Molecular Formula: C2H6OS Molecular Weight: 78.13 Purity/Analysis Method: >99.0% (GC) Form: Clear Liquid Boiling point (°C): 158 Melting point (°C): -100 Flash Point (°C): 74 Specific Gravity (20/20): 1.12
Supplier:
MP Biomedicals
Description:
2-Merceptoethanol is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. 2-Merceptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis. 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.
Supplier:
THERMO FISHER SCIENTIFIC CHEMICALS
Description:
2-Mercaptoethanol 99%, pure
Supplier:
MP Biomedicals
Description:
β-Mercaptoethanol can act as an enzyme reactivator in systems necessitating reduction for activation, and has been commonly used to reduce disulfide bonds in order to separate protein subunits for use in electrophoresis.
2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-Mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-Mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.
Catalog Number:
(80501-154)
Supplier:
MilliporeSigma
Description:
β-Mercaptoethanol, Molecular Biology Grade a disulfide-reducing agent.
Supplier:
MP Biomedicals
Description:
Storage: Room Temperature
2-Mercaptoethanol is a hybrid of ethylene glycol and 1,2-ethanedithiol. It is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. It is widely used because the hydroxyl group confers solubility in water and lowers the volatility. Due to its diminished vapor pressure, its odour, while unpleasant, is less objectionable than related thiols. 2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also it reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-Mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-Mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.
Supplier:
Thermo Scientific Chemicals
Description:
Solubilizes proteins by reducing disulfide linkages
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