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The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.
Description:
<i>Pfu</i> DNA ligase is a recombinant enzyme derived from <i>Pyrococcus furiosus</i> hyperthermophilic marine archaebacterium that catalyzes linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45 to 80 °C, with a temperature optimum near 70 °C for nick-sealing reactions.
Description:
The Select DNA cloning and amplification kits generate a minimum of 10 µg of amplified DNA from fragments of 0.3 to 1.8 kb using the BioXp® 3250.
Description:
The Select DNA cloning Golden Gate Assembly BsmBI kits are compatible with the Type IIS restriction enzymes BsmBI. Please choose the appropriate kit based on the number of fragments being assembled.
Description:
The Select DNA cloning golden gate assembly, BsaI kits are compatible with the Type IIS restriction enzymes BsaI. Please choose the appropriate kit based on the number of fragments being assembled.
Description:
This PCR Cloning Kit contains an optimized Cloning Mix containing a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background.
Description:
The Select DNA cloning and amplification kits generate a minimum of 10 µg of amplified DNA from fragments of 0.3 to 1.8 kb using the BioXp 9600.
Description:
This NEB Golden Gate Assembly Kit (BsaI-HFv2) contains an optimized mix of BsaI-HFv2 and T4 DNA Ligase. Together these enzymes can direct the assembly of multiple inserts/modules using the Golden Gate approach. The pGGA destination plasmid (included), provides a backbone for your assembly, features convenient restriction enzyme sites for subcloning, and has T7/SP6 promoter sequences to enable <i>in vitro</i> transcription.
Description:
The GeneMorph II random mutagenesis kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis.
Description:
The StrataClone PCR Cloning Kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase.
Description:
The Select DNA cloning Gibson assembly kits can generate Gibson clones starting with fragments and vector supplied by the end user.
New Product
,77506-808EA,77506-808EA,77506-810EA,77506-810EA
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
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The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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