DSP (Dithiobis(succinimidyl propionate))
Supplier:
TCI America
Description:
[Cross-linking Reagent]
CAS Number: 57757-57-0 MDL Number: MFCD00042045 Molecular Formula: C14H16N2O8S2 Molecular Weight: 404.41 Purity/Analysis Method: >97.0% (N) Form: Crystal Melting point (°C): 133 Storage Temperature: 0-10°C
Catalog Number:
(82022-782)
Supplier:
G-Biosciences
Description:
Dithiobis(succinimidyl propionate) Homobifunctional Cross Linker.
Supplier:
AMBEED, INC
Description:
Di(N-succinimidyl) 3,3-Dithiodipropionate [Cross-linking Reagent], Purity: 97%, CAS Number: 57757-57-0, Appearance: White to off-white solid, Storage: Inert atmosphere, 2-8 C, Size: 250mg
Catalog Number:
(103523-924)
Supplier:
Invitrogen
Description:
Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.
Supplier:
Invitrogen
Description:
Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.
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