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Coelenterazine Substrates for Renilla Luciferase and Apoaequorin, Biotium

Supplier: BIOTIUM, INC
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89138-290EA 82.6 USD InStock
89138-290 89138-292 89138-294 89138-296 89138-298 89138-300 89138-302 89138-304 89138-306 89138-308 89138-310 89138-312 89138-314 89138-316 89138-318 89138-320 89138-322 89138-324 89138-326 89138-328 89138-330 89138-332 89138-334 89138-336 89138-338 89138-340 89138-342 89138-344 89138-346 89138-348 89138-350 89138-352 89410-672 89411-152 89411-156 89493-036 89493-038 75832-546 75832-548
Coelenterazine Substrates for Renilla Luciferase and Apoaequorin, Biotium
Protein Linking and Labeling Systems
Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.

Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase. Apoaequorin is used as a calcium indicator whereas Renilla luciferase is commonly used as a reporter of transcription regulation. Coelenterazine binds to apoaequorin to form aequorin which emits light upon binding to calcium. Aequorin can be used to measure a broad concentration range of calcium from ~0.1 uM to >100 uM. Renilla luciferase has been widely used as a reporter protein and as a bioluminescence donor in bioluminescence resonance energy transfer (BRET) to study protein-protein interactions. Other uses of coelenterazine include chemiluminescent detection of superoxide anion and peroxynitrite in cells or tissues.

Can’t decide which coelenterazine analog to use? Try the Coelenterazine Sampler Kit (catalog no. 89138-348). The kit includes 25 ug each of nine coelenterazine analogs including native, cp, f, fcp, h, hcp, i, ip and n.

There are over a dozen of coelenterazine analogs that have been synthesized, many of which are now commercially available from Biotium. These coelenterazine analogs have different properties in terms of emission wavelength, cell membrane permeability and quantum efficiency.

Biotium offers coelenterazine 400a (also known as DeepBlue C™) (catalog no. 89138-350) which serves as a substrate for Renilla luciferase (Rluc) and generates an emission peak centered around 400 nm. It is the preferred Renilla luciferase substrate for BRET studies because it has minimal interference with the emission of the GFP acceptor.
Also, see Biotium's water soluble Aquaphile™ Coelenterazines, sold as coelenterazine native (catalog no. 89411-152) or coelenterzine h analog (catalog no. 89411-156). These formulations readily dissolve in water or buffer for in vivo use.
Lastly, Biotium offers methyl coelenterazine (coelenterazine, 2-methyl analog) (catalog no. 89138-344) which has been reported to be a superior antioxidant for cells against reactive oxygen species (ROS) such as singlet oxygen and superoxide anion. The coelenterazine derivative is membrane-permeant, non-toxic and highly reactive toward ROS.


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