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2-[Bis(2-hydroxyethyl)amino]ethanesulfonic acid ≥99%, white crystalline powder

Supplier: E. Becker & Co. GmbH
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0210092705 0210092780 0210092783 0210092791 0210092725
IC10092705EA 46.34 USD InStock
IC10092705 IC10092780 IC10092783 IC10092791 IC0210092725
2-[Bis(2-hydroxyethyl)amino]ethanesulfonic acid ≥99%, white crystalline powder
2-[Bis(2-hydroxyethyl)amino]ethanesulphonic acid
BES is a zwitterionic buffer that is used in biochemistry and molecular biology research in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies.

  • Presentation: White powder

A protocol has been reported for the use of BES buffered saline in the calcium phosphate mediated transfection of eukaryotic cells with plasmid DNA. BES, a sulfonic acid-containing cross-linking agent, induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes.

The effect of BES and other zwitterionic buffers on the Bicinchoninic acid (BCA) measurement of microgram quantities of protein has been studied. BES was found to diminish color development of the protein BCA complex by 30-35% at 50 mM, and by 10% at 5 mM, measuring in the 1-10 mg range of BSA against protein-BCA complexes formed in water. This color loss may result from binding of Cu2+ to BES.

BES may be employed as binding buffer in modified Eagle′s medium during the binding assay of human melanoma cells. It may be used as biobuffer to investigate the aqueous medium self-assembly of heterometallic CuII/Li 3D coordination polymers.

Soluble 0.1M in water (clear, colorless solution)

BES is a zwitterionic buffer that is used in biochemistry and molecular biology research in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. A protocol has been reported for the use of BES buffered saline in the calcium phosphate mediated transfection of eukaryotic cells with plasmid DNA


Formula: C₆H₁₅NO₅S
Melting Pt: 152…156 °C
Storage Temperature: Ambient
 MDL Number: MFCD00007533
CAS Number: 10191-18-1
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Specification Test Results
Identity Test Passes
FTIR Conforms to Standard
pH ( 1% aq soln) 3 - 5
Assay (titration, dry basis) ≥99.0%
Solubility (0.1M in H₂O) Clear, Complete
Moisture ≤1.0%
pKa 6.9 - 7.3
UV A260 nm ≤0.10
UV A280 nm ≤0.08

 

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