0210095501
0210095505
0210095510
IC10095501EA
69.71
USD
InStock
IC10095501
IC10095505
IC10095510
5-Methylphenazinium methyl sulphate, dark yellow powder
5-Methylphenazinium methyl sulphate
Phenazine Methosulfate is an electron acceptor and carrier in enzyme systems. The oxidized form is yellow and the reduced form is colorless.
Phenazine methosulfate is used in tyrosine transaminase test and for enzymic determination of ethanol in blood by the colorimetric micromethod. Used with ascorbic acid to determine nitric oxide reductase activity. Since the reduced PMS is easily oxidized by oxygen, it is used in assays as an electron carrier between enzymes and oxygen, cytochrome c, indophenols, or tetrazolium salts. The reduced PMS is used as an electron donor to reduce cytochrome c or in photosynthetic experiments. Usage of PMS for detection of specific dehydrogenases has been reported.
PMS is reduced by flavoproteins such as succinic dehydrogenase. PMS is reduced non-enzymatically by NADH and NADPH. PMS is also reduced by dithionite, sodium borohydride, ascorbic acid, reduced ubiquinones, and reduced vitamin K. PMS reacts with sulfhydryl groups including those of enzymes.
Soluble in water(800 mg/4 mL), ethanol,methanol (5% w/v); insoluble in benzene, diethyl ether.
PMS is reduced by flavoproteins such as succinic dehydrogenase. PMS is reduced non-enzymatically by NADH and NADPH. PMS is also reduced by dithionite, sodium borohydride, ascorbic acid, reduced ubiquinones, and reduced vitamin K. PMS reacts with sulfhydryl groups including those of enzymes.