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N,N,N',N'-Tetramethylethylenediamine (TEMED) ≥99%, liquid for electrophoresis

Supplier: E. Becker & Co. GmbH

04805615 04805613 04805614
IC805615EA 109.58 USD InStock
IC805615 IC805613 IC805614
N,N,N',N'-Tetramethylethylenediamine (TEMED) ≥99%, liquid for electrophoresis
N,N,N',N'-Tetramethylethylenediamine (TEMED)
N,N,N',N'-Tetramethylethylenediamin is a tertiary amine base.

N,N,N',N'-Tetramethylethylenediamine is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules such as nucleic acids and proteins. TEMED has been used in the coating for soft polymers, such as polyacrylamide, in capillaries for investigation by atom force microscopy. Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED. It is also used used in the HPLC of small organic molecules.

Easily soluble in water (20 gm/mL-clear-cololess solution). Suggested use is 6-10 uL for 10 mL acrylamide gel solution (the less amount of TEMED used, the slower the polymerization)

Suitable for Polyacrylamide Gel Electrophoresis


Formula: C₆H₁₆N₂
MW: 116.21 g/mol
Boiling Pt: 120
Melting Pt: -64
Flash Pt: 17
 MDL Number: MFCD00008335
CAS Number: 110-18-9
UN: 2372
ADR: 3 II
https://qa.digitalassets.avantorsciences.com/adaptivemedia/rendition?id=34fbd658a9b100ea38108e497591bb43269b1833&vid=34fbd658a9b100ea38108e497591bb43269b1833&prid=web&clid=SAPDAM
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Specification Test Results
Synonyms 1,2-Bis(dimethylamino)ethane; N,N,N′,N′-Tetramethylethylenediamine; TMEDA
Form Liquid
Storage Store at Room Temperature (15-30 °C), store under nitrogen.
Moisture Content ≤2%
pH 10 - 12 (0.5% aq soln)
Refractive Index n20/D 1.4179 (Lit.)

 

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