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Pierce™ EZ-Link® Sulfhydryl Reactive Biotinylation Reagents, Thermo Scientific

Supplier: Paul Schöndorf Metallwaren GmbH
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21333 21334 21341 21900 21901BID 21911
PI21333EA 499.91 USD InStock
PI21333 PI21334 PI21341 PI21900 PI21901 PI21911
Pierce™ EZ-Link® Sulfhydryl Reactive Biotinylation Reagents, Thermo Scientific
Protein Linking and Labeling Systems
Sulfhydryl groups are the second most common target for modification in biological studies. They are found as exposed cysteines in protein and peptides. Sulfhydryl group biotinylation provides an advantage in some applications; for example, targeting a sulfhydryl group can help preserve the biological activity of an enzyme when amines are found at the active site.

Maleimide-PEG2 and PEG11-Biotin: Water-soluble

Biotin-BMCC: Reacts with sulfhydryl (-SH) groups at 6.5 to 7.5 pH. Avoids potential modification of tyrosine residues that can be associated with Iodoacetyl-Biotin. Must be dissolved in DMSO or DMF before use.

Iodoacetyl-PEG2-Biotin: Water soluble. Reacts with sulfhydryl (-SH) groups at basic to neutral pH. Coupling reactions occur in the dark.

Iodoacetyl-LC-Biotin: Reacts with sulfhydryl (-SH) groups at basic to neutral pH. Coupling reactions occur in the dark. Must be dissolved in DMSO or DMF before use.

Reacts with sulfhydryl (-SH) groups at acidic to neutral pH. PEG11-Biotin has an extra long spacer arm that reduces steric hindrance.


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