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Chloroform ≥99.8% ACS

Supplier: E. Becker & Co. GmbH

0219381404 0219381490 0219381491
IC0219381404EA 323.27 USD InStock
IC0219381404 IC0219381490 IC0219381491
Chloroform ≥99.8% ACS
Chloroform
Chloroform is typically used as a solvent and as a cleansing agent. Procedures have been described for the use of chloroform in lambda plaques storage, lambda cDNA storage, the removal of mineral oil from PCR reaction samples, oligonucleotide purification, a hydroxyl radical footprinting protocol, a transcriptional run-on assay protocol, and an overlay assay for beta-galactosidase activity. It has also been used with phenol in such procedures as DNA recovery from polyacrylamide gels, ethidium bromide removal from DNA preparations, lysis protocols for plasmid DNA isolation, RNase removal, and purification of yeast DNA. A protocol describes the use of chloroform in a high-performance thin-layer chromatography protocol for sphingomyelin analysis. Chloroform can also be used in chloroform/methanol mixtures for the isolation of cardiolipids from Geobacillus stearothermophilus and their subsequent MS analysis. The isolation of the bacteriocin amylovorin L471 from Lactobacillus amylovorus DCE 471 in culture broth has been reported, using chloroform/methanol extraction and precipitation in the procedure. Chloroform/2-butanol mixtures can be used for the extraction of steroid sulfates for analysis by nanoelectrospray ionization mass spectrometry.


Formula: CHCl₃
MW: 119.38 g/mol
Boiling Pt: 61.7 (lit.)
Melting Pt: -63 (lit.)
Density: 1.476…1.478 g/cm³ (20 °C) (lit.)
Storage Temperature: Ambient
 MDL Number: MFCD00000826
CAS Number: 67-66-3
EINECS: 200-663-8
UN: 1888
ADR: 6.1 III
Merck Index: 13,02160
https://qa.digitalassets.avantorsciences.com/adaptivemedia/rendition?id=034568d3c85846f7cda5b84377449e8a3642315c&vid=034568d3c85846f7cda5b84377449e8a3642315c&prid=web&clid=SAPDAM
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Specification Test Results
Physical Appearance Liquid
Auto Ignition Above 1000 °C(Lit.)
Vapor Density 4.36 (air = 1)(Lit.)
Vapor Pressure 211 hPa at 20 °C(Lit.)
Refractive Index n20/D 1.445(lit.)
UV/Visible Absorbance λ: 245 nm Amax: 1.00, λ: 255 nm Amax: 0.25, λ: 260 nm Amax: 0.15, λ: 270 nm Amax: 0.05, λ: 290-400 nm Amax: 0.01(Lit.)
Color APHA ≤10
Solubility Soluble in water (1 mL/200 mL water); miscible with ethanol, benzene, ether, petroleum ether, carbon tetrachloride, carbon disulfide, oils.

 

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