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Update to Avantor’s response to the coronavirus (COVID-19) pandemic

5-Fluoroorotic acid monohydrate (5-FOA) ≥98%, powder

Supplier: SmartPractice Europe
Warning

F9001-1 F9001-5
77001-952EA 66.56 USD InStock
77001-952 77001-954
5-Fluoroorotic acid monohydrate (5-FOA) ≥98%, powder
5-Fluoroorotic acid monohydrate (5-FOA)
Yeast genetic counter-selection agent.

  • Available as an ultra-pure powder ( 98% purity) or as a solution in DMSO

Using 5-Fluoroorotic Acid (5-FOA) for the counter-selection of yeast is a common genetic screening method. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. Otherwise nontoxic to yeast, 5-FOA is converted to the toxic form (i.e., 5-flurouracil) in strains expressing the functional URA3 gene coding for orotine-5'-monophosphate decarboxylase that is involved in the synthesis of uracil. Yeast strains that are phenotypically Ura+ become Ura- and 5-FOAR after selection. The question of 5-FOA solubility is often raised by researchers using ultra-pure ( 98%) 5-FOA powder because of its insolubility in water. Thus, we provide a 100X concentrated (100 mg/mL) 5-FOA solution in DMSO. This has been tested and validated on the basis of counter selection activity.


Formula: C₅H₃FN₂O₄·1H₂O
Storage Temperature: Freezer
 MDL Number: MFCD00150658
CAS Number: 220141-70-8
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