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Extracta Plus RNA Kits, Quantabio

Supplier: POLY SERVICE BV
Extracta Plus RNARapid extraction and purification of high-quality total RNA from cultured cells or tissue
Extracta Plus RNA procedure.This procedure can be completed in just 25 mins, resulting in ready-to-use total RNA applicable for sensitive downstream applications such as RT-qPCR and NGS.
Total RNA yield & negligible gDNA yield. PCR assays for human or rat ?-actin were performed. A standard curve of human/rat gDNA was used to quantify the amount of gDNA contamination in the purified RNA. For all samples, gDNA was present in negligible quantities.
Extraction of intact high molecular weight RNA from tissues and cultured cells.Total RNA purified of rat kidney or lung tissue or from 1X106 cultured HeLa cells (H) using Extracta Plus RNA Kit and amplified via RT-PCR using qScript µltra Flex Kit and repliQa HiFi Toughmix.
Typical yields from various input samples.
Quantitative extraction of RNA from dilute cell quantities.Total RNA purified from HeLa cell from 100,000 to 10 cells using the Extracta Plus RNA Kit. RNA (1.5 ?l) was used for qPCR analysis of ?-actin mRNA using qScript XLT ToughMix.
Effective gDNA removal from cells and tissue.Total RNA was purified from A 1 x 106 HeLa cells or B 10 mg rat kidney tissue using the Extracta Plus RNA Kit. RT-qPCR assays were performed with (+RT) or without (–RT) reverse transcriptase.
Extracta Plus RNARapid extraction and purification of high-quality total RNA from cultured cells or tissue
Extracta Plus RNA procedure.This procedure can be completed in just 25 mins, resulting in ready-to-use total RNA applicable for sensitive downstream applications such as RT-qPCR and NGS.
Total RNA yield & negligible gDNA yield. PCR assays for human or rat ?-actin were performed. A standard curve of human/rat gDNA was used to quantify the amount of gDNA contamination in the purified RNA. For all samples, gDNA was present in negligible quantities.
Extraction of intact high molecular weight RNA from tissues and cultured cells.Total RNA purified of rat kidney or lung tissue or from 1X106 cultured HeLa cells (H) using Extracta Plus RNA Kit and amplified via RT-PCR using qScript µltra Flex Kit and repliQa HiFi Toughmix.
Typical yields from various input samples.
Quantitative extraction of RNA from dilute cell quantities.Total RNA purified from HeLa cell from 100,000 to 10 cells using the Extracta Plus RNA Kit. RNA (1.5 ?l) was used for qPCR analysis of ?-actin mRNA using qScript XLT ToughMix.
Effective gDNA removal from cells and tissue.Total RNA was purified from A 1 x 106 HeLa cells or B 10 mg rat kidney tissue using the Extracta Plus RNA Kit. RT-qPCR assays were performed with (+RT) or without (–RT) reverse transcriptase.

95214-010 95214-050
76492-574EA 78.57 USD
76492-574 76492-576
Extracta Plus RNA Kits, Quantabio
Nucleic Acid Reagents Nucleic Acid Purification Kits and Reagents

Rapid extraction and purification of high-quality total RNA from cultured cells or tissue.