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PureCube Glutathione Agarose

Supplier: CUBE BIOTECH

32115 32103 32105 32110 32112
77393-044PK 14584.84 USD InStock
77393-044 77393-034 77393-036 77393-038 77393-040 77393-042
PureCube Glutathione Agarose
Protein Purification Protein Purification Reagents
The protein purification with the PureCube Glutathione Agarose and the GST-tag exploit the specific enzyme-substrate binding to gain high levels of purity while maintaining a high protein yield.

  • Usage: Specific binding and purification of GST-tagged proteins
  • Specificity: Affinity to GST(Glutathione-S-Transferase)-tagged proteins
  • Binding capacity: >10 mg/ml
  • Bead Ligand: L-Glutathione (reduced)
  • Bead size: 40 μm
  • Matrix: 7.5% cross-linked agarose
  • Filling quantity: Delivered as a 50% suspension
  • pH Stability: 3 to 12
  • Required equipment:
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 ml tube (min 10000×g)
  • 50 ml centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  • pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment

The pairing of an enzyme to its substrate is one of the most specific combinations in life sciences. PureCube Glutathione Agarose is a suspension of tiny 40 µm purification beads that are used for this purpose. Their small size provides high mechanical stability. They can be used for all sorts of affinity chromatography methods, such as FPLC, batch spin, or column chromatography. You can also purchase these agarose beads pre-packed into FPLC-ready cartridges. Additionally, we offer GST-tag affinity resin with a bead diameter of 400 µm for very viscous cell media and higher FPLC flow rates. Alternatively, Glutathione MagBeads are also available.


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