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Update to Avantor’s response to the coronavirus (COVID-19) pandemic

PureCube Ni-NTA Agarose

Supplier: CUBE BIOTECH

31103 31105 31110 31112 31115
77392-952EA 172.42 USD InStock
77392-952 77392-954 77392-956 77392-958 77392-960 77392-950
PureCube Ni-NTA Agarose
Protein Purification Protein Purification Reagents
PureCube Ni-NTA agarose resins are your best option to purify His-tagged protein using FPLC, Batch spin, or column chromatography.

  • Usage: Specific binding and purification of 6× His tagged proteins
  • Specificity: Affinity to His tagged proteins
  • Binding capacity: 20 to 80 mg/ml, depending on bead size
  • Bead ligand: Ni-NTA
  • Bead size: 40 μm, 100 µm, or 400 µm
  • Chelator stability: Stable in buffer containing 10 mM DTT and 1 mM EDTA
  • Filling quantity: Delivered as a 50% suspension

PureCube Ni-NTA agarose size ranges from market standard 40 µm and 100 µm diameters to 400 µm (XL). These three options provide plenty of possibilities to choose the correct agarose resin for your purpose. Choose smaller beads for higher mechanical stability and higher protein yield. Meanwhile, bigger beads provide faster flow rates and the possibility of working with very viscous cell media.

Required equipment:
-Lysis buffer
-Wash buffer
-Elution buffer
-Ice bath
-Refrigerated centrifuge for 50 ml tube (min 10000×g)
-50 ml centrifuge tube
-Micropipettor and Micropipetting tips
-Disposable gravity flow columns with capped bottom outlet, 2 ml
-pH meter
-End-over-end shaker
-SDS-PAGE buffers, reagents, and equipment; Optional: Western Blot reagents and equipment


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