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X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside), white powder Molecular biology grade

Supplier: E. Becker & Co. GmbH

0215000105 0215000150 0215000180 0215000183 0215000190 0215000191 0215000110
IC15000105EA 52.87 USD InStock
IC15000105 IC15000150 IC15000180 IC15000183 IC15000190 IC15000191 75875-614
X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside), white powder Molecular biology grade
X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)
5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside, commonly known as X-Gal, is a histochemical substrate for b-galactosidase. X-Gal is cleaved by b-galactosidase to yield an insoluble blue precipitate.

X-Gal is particularly useful in molecular biology applications to detect the activity of b-galactosidase which is frequently used as a reporter gene. Insertion of DNA into the lac Z region results in the loss of b-galactosidase activity. Lac+ bacterial colonies resulting from a-complementation will appear blue whereas bacterial colonies containing plasmid with DNA inserted in the lac Z region, will be incapable of a-complementation and will appear white.

5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside is used as indigogenic substrate for b - galactosidase, for detection of b - galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.


Formula: C₁₄H₁₅BrClNO₆
Melting Pt: 230 °C (dec.)
Storage Temperature: Freezer
 MDL Number: MFCD00005666
CAS Number: 7240-90-6
EINECS: 230-640-8
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