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3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate (CHAPS) ≥98%, white powder

Supplier: E. Becker & Co. GmbH
Warning

0219031901 0219031905 0219031925 0219031980
IC19031901EA 86 USD InStock
IC19031901 IC19031905 IC19031925 IC0219031980
3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate (CHAPS) ≥98%, white powder
3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate (CHAPS)
CHAPS is a nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents.

  • pH: 4.0 - 7.0 (10% aq soln)
  • Aggregation Number: 4-14 (0-0.1 M Na+)
  • Critical Micelle Concentration (CMC): 6-10 mM

CHAPS is commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing. CHAPS has been shown to give excellent resolution of some subcellular preparations and plant proteins. Its small micellar molecular weight (6150) and high CMC (6-10 mM) allow it to be removed from samples by dialysis. Can be used as a non-toxic stabilizing agent for growth factors.

CHAPS is commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing. CHAPS has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 1-4% (v/v) are typically used in an isoelectric focusing gel. A commonly used isoelectric focusing sample solution consists of 8 M urea, 4% CHAPS, 50-100 mM dithiothreitol (DTT) and 40 mM Tris.4 Its small micellar molecular weight (6150) and high CMC (6-10 mM) allow it to be removed from samples by dialysis. Can be used as a non-toxic stabilizing agent for growth factors.

Soluble in water (100 mg/ml - clear, colorless to slightly yellow solution).

Precaución: Research Use Only (RUO).


Formula: C₃₂H₅₈N₂O₇S
MW: 614.89 g/mol
Melting Pt: 116 (lit.)
Storage Temperature: Ambient
 MDL Number: MFCD00081083
CAS Number: 75621-03-3
Merck Index: 13,02052
https://qa.digitalassets.avantorsciences.com/adaptivemedia/rendition?id=e1fe6360a3dd91d2a4d7755a838fa2e97d4bce2e&vid=e1fe6360a3dd91d2a4d7755a838fa2e97d4bce2e&prid=web&clid=SAPDAM
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Specification Test Results
Identity Test Passes
Purity ≥98%
FTIR Conforms to Standard
OD400 (5% aq soln) Report Result
OD290 (5% aq soln) Report Result

 

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