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TRIS HCl (tris(hydroxymethyl)aminomethane hydrochloride)

Supplier: Bioanalytic
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T60050-100.0 T60050-1000.0 T60050-250.0 T60050-25000.0 T60050-500.0 T60050-5000.0 T60050-10000.0
76348-660EA 52.28 USD InStock
76348-660 76348-662 76348-664 76348-666 76348-668 76348-670 77510-478
TRIS HCl (tris(hydroxymethyl)aminomethane hydrochloride)
TRIS HCl (tris(hydroxymethyl)aminomethane hydrochloride)
TRIS Hydrochloride, Tris-HCl, is a buffering agent commonly utilized to maintain a stable pH in experimental procedures.

It is primarily used as a buffering agent for applications that require a slightly alkaline to neutral pH range. Also, It is effective in the pH range of 7.2 to 9.0, making it suitable for a variety of biochemical and molecular biology techniques. TRIS HCl is commonly used in DNA and RNA extraction, protein purification, enzyme assays, and electrophoresis. It helps maintain the desired pH conditions for enzymatic reactions, stability of nucleic acids, and separation of proteins during electrophoresis. One of the advantages of TRIS HCl is its ability to resist changes in pH caused by the addition of acids or bases, making it a reliable buffering agent. It also exhibits low ionic strength, which minimizes interference with biological systems and enzymatic activities.

Precaución: Research or further manufacturing use only, not for food or drug use.


Formula: NH₂C(CH₂OH)₃·HCl
Melting Pt: 150...153 °C
Storage Temperature: Room Temperature
 MDL Number: MFCD00012590
CAS Number: 1185-53-1
EINECS: 214-684-5
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Specification Test Results
Appearance White crystalline
Assay 99.0 - 101.0%
pH (1% aqueous solution) 4.2 - 5.0
Solubility (1M Solution in Water) Clear, colorless solution
UV Absorbance at 260 nm, 1.0 M in H₂O ≤0.06
UV Absorbance at 280 nm, 1.0 M in H₂O ≤0.05
Identification Conforms
Heavy Metals ≤5 ppm
Grade USP
BSE/TSE Free BSE/TSE Free
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