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Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)

Supplier: Bioanalytic
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T62500-5000.0 T62500-1000.0
76348-720EA 294.12 USD InStock
76348-720 77510-486
Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)
Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)
TRIS, also known as tris(hydroxymethyl)aminomethane, is a chemical compound widely used in life science as a buffer. TRIS is commonly employed in biological and biochemical research to maintain a stable pH in various experimental procedures. It is valued for its buffering capacity and its ability to maintain a constant pH in a desired range.

It is commonly used in applications such as protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques. TRIS is highly effective in buffering solutions in the pH range of 7.0 to 9.2, making it suitable for a wide range of biological processes and experimental conditions. It is particularly useful for maintaining a stable pH in the alkaline range. One of the significant advantages of TRIS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.


Formula: NH₂C(CH₂OH)₃
Melting Pt: 171...172 °C
Storage Temperature: Room Temperature
 MDL Number: MFCD00004679
CAS Number: 77-86-1
EINECS: 201-064-4
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Specification Test Results
Appearance White Crystalline Powder
Absorbance at 280 nm (1M Solution) ≤0.05
Absorbance at 300 nm (25% Solution) ≤0.04
APHA Color, 20% Aqueous solution ≤25
Arsenic (As) ≤5 ppm
Assay (Titration, dried basis) 99.5 - 101.0%
Chloride ≤1 ppm
Copper (Cu) ≤1 ppm
DNase, RNase, protease Not Detected
Electrophoresis Pass Test
Heavy Metals (as Pb) ≤1 ppm
Identification (IR) Pass
Iron (Fe) ≤1 ppm
Magnesium (Mg) ≤10 ppm
Melting Point 168.0 - 172.0 °C
Moisture (KF) ≤0.5%
pH (5% Solution) 10.0 - 11.5
Residue on Ignition ≤0.1%
Solubility (40% Aqueous Solution) Clear
Sulfate ≤5 ppm
Turbidity (40% Solution) ≤2.0
UV Absorbance at 260 nm, 10% Aqueous solution ≤0.1
UV Absorbance 280 nm (10% Solution in water) ≤0.08
UV Absorbance at 430 nm, 10% Aqueous solution ≤0.009
BSE/TSE Free BSE/TSE Free
Non Animal Origin Non Animal Origin
Penicillin or other Beta Lactams None Present
Country of Origin Report

 

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