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Pierce™ BCA® Protein Assay Kits and Reagents, Thermo Scientific

Supplier: Paul Schöndorf Metallwaren GmbH
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23209 23212 23208 23210 23213 23221 23222 23223 23224 23225 23227 23228 23230
PI-23209PK 108.69 USD InStock
PI-23209 PI-23212 PI23208 PI23210 PI23213 PI23221 PI23222 PI23223 PI23224 PI23225 PI23227 PI23228 PI23230
Pierce™ BCA® Protein Assay Kits and Reagents, Thermo Scientific
Assays Protein Assays
Pierce BCA™ (bicinchoninic acid) reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA™ Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA™ Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

  • Colorimetric, so can measure with a standard spectrophotometer or plate reader (562 nm)
  • Excellent uniformity, exhibiting less protein-to-protein variation than dye-binding methods
  • Unaffected by typical concentrations of most ionic and nonionic detergents
  • Easier and faster than the classic Lowry method
  • High linearity and sensitivity—linear working range for BSA 20 to 2000 µg/mL; detection down to 5 µg/m (with the enhanced protocol)

The assay kits can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications

The BCA protein assay combines the well-known reduction of Cu2+ to Cu1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu1+) by bicinchoninic acid. The first step is the chelation of copper with protein in an alkaline environment to form a light blue complex. In this reaction, known as the biuret reaction, peptides containing three or more amino acid residues form a colored chelate complex with cupric ions in an alkaline environment containing sodium potassium tartrate.

In the second step of the color development reaction, bicinchoninic acid (BCA) reacts with the reduced (cuprous) cation that was formed in step one. The intense purple-colored reaction product results from the chelation of two molecules of BCA with one cuprous ion. The BCA/copper complex is water-soluble and exhibits a strong linear absorbance at 562nm with increasing protein concentrations. The BCA reagent is approximately 100 times more sensitive (lower limit of detection) than the pale blue color of the first reaction.

The reaction that leads to BCA color formation is strongly influenced by four amino acid residues (cysteine or cystine, tyrosine, and tryptophan) in the amino acid sequence of the protein. However, unlike the Coomassie dye-binding methods, the universal peptide backbone also contributes to color formation, helping to minimize variability caused by protein compositional differences.

Información de suministro: Each Pierce BCA™ protein assay kit contains BCA Reagent A (500 mL or 2x500 mL), BCA Reagent B (25 mL), and Albumin Standard Ampoules (2 mg/mL, 10x1 mL).


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