3,5-Difluoro-2-nitrobenzoic+acid
Catalog Number:
(82022-846)
Supplier:
G-Biosciences
Description:
Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow colored product at 412nm.
Ellman's reagent is very useful as a free sulfhydryl assay reagent due to its high specificity for -SH groups at neutral pH, high molar extinction coefficient and short reaction time.
Supplier:
AMBEED, INC
Description:
Methyl 2-chloro-4-nitrobenzoate, Purity: 98%, CAS Number: 13324-11-3, Appearance: White to Orange to Green powder to crystal, Storage: Sealed in dry, Room Temperature, Size: 25g
Supplier:
Matrix Scientific
Description:
Matrix Scientific Part Number: 047997-500MG , MDL Number: MFCD00272139
Supplier:
Matrix Scientific
Description:
Matrix Scientific Part Number: 051772-500MG , MDL Number: MFCD13561788
Supplier:
AMBEED, INC
Description:
5-Nitro-1-benzothiophene-2-carboxylic acid 96%
Supplier:
Matrix Scientific
Description:
Matrix Scientific Part Number: 045139-5G , MDL Number: MFCD00184587
Supplier:
Thermo Scientific Chemicals
Description:
25g CAS: 55737-66-1, MDL: MFCD06203344
Supplier:
Matrix Scientific
Description:
6-Nitro-m-anisic acid ≥95%
Supplier:
Matrix Scientific
Description:
2-(16,18-Dioxo-17-azapentacyclo[6.6.5.0~2,7~.0~9,14~.0~15,19~]nonadeca-2,4,6,9,11,13-hexaen-17-yl)-4-nitrobenzoic acid
Supplier:
Matrix Scientific
Description:
5-Bromo-3-nitro-o-toluic acid ≥95%
Supplier:
AMBEED, INC
Description:
4-Bromo-3,5-difluorophenol, Purity: 98%, CAS Number: 130191-91-2, Appearance: Solid or Semi-solid or liquid or lump, Storage: Inert atmosphere, Room Temperature, Size: 100g
Supplier:
Matrix Scientific
Description:
3-Nitrosalicylic acid ≥97%
Supplier:
AMBEED, INC
Description:
2-Hydroxy-4-nitrobenzoic acid, Purity: 97%, CAS Number: 619-19-2, Appearance: White to yellow to greyish yellow red or gray powder or crystals, Storage: Sealed in dry, Room Temperature, Size: 10g
Supplier:
BeanTown Chemical
Description:
CAS: 96-98-0; EC No: 202-549-3; MDL No: MFCD00007174
Crystalline; Molecular Formula: C8H7NO4; MW: 181.15
Melting Point: 187-190°
Supplier:
Adipogen
Description:
A sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues. Used to characterize reactive thiol groups and photometric determination of thiols and for measuring low-molecular mass thiols such as glutathione in both pure solutions and biological samples, such as blood. It can also measure the number of thiol groups on proteins. Through reaction with aliphatic thiol groups a mixed disulfide of protein thiol and one mole of 2-nitro-5-thiobenzoate per mole of protein sulfhydryl group is being formed. DTNB has little absorbance. Reaction with -SH groups on proteins (from any solvent accessible Cys) under mild alkaline conditions (pH 7-8) produces the 2-nitro-5-thiobenzoate anion, which gives an intense yellow color with an absorption maximum at 409.5nm (Extinction coefficient: 14150 M-1*cm-1). Sensitive to various buffer ions, therefore, the extinction coefficient used to calculate the number of sulfhydryl groups must be matched to the reaction conditions. In case the thiol groups are in disulfide bonds, they must be reduced under anaerobic conditions prior to reaction with DTNB.
Catalog Number:
(77280-168)
Supplier:
AMBEED, INC
Description:
4-(Methoxycarbonyl)-2-nitrobenzoic acid, Purity: 95%, CAS Number: 55737-66-1, Appearance: Solid or liquid, Storage: Sealed in dry, Room Temperature, Size: 1g
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