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6-Carboxyfluorescein
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6-Carboxyfluorescein
Supplier:
Bachem Americas
Description:
Sequence: 6-Carboxy-fluorescein
Synonym(s): 6-FAM#2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-terephthalic acid
Supplier:
Adipogen
Description:
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is a useful fluorescent tracer that can passivley diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.
Catalog Number:
(76483-504)
Supplier:
AAT BIOQUEST INC
Description:
It is widely recognized that fluorescent labeling of cells is an effective means to determine total cell numbers or how many viable cells exist in a sample.
Catalog Number:
(76483-496)
Supplier:
AAT BIOQUEST INC
Description:
It is widely recognized that fluorescent labeling of cells is an effective means to determine total cell numbers or how many viable cells exist in a sample.
Catalog Number:
(89139-240)
Supplier:
Biotium
Description:
BCECF (2′,7′ –bis-(Carboxyethyl)-5-(and-6)-carboxyfluorescein) is the most widely used fluorescent pH sensor. Because its pKa (6.97) is close to physiological pH, BCECF can detect cytosolic pH change with high sensitivity.
Catalog Number:
(76483-498)
Supplier:
AAT BIOQUEST INC
Description:
It is widely recognized that fluorescent labeling of cells is an effective means to determine total cell numbers or how many viable cells exist in a sample.
Catalog Number:
(89139-558)
Supplier:
Biotium
Description:
5(6)-FAM SE (full name: 5-(and-6)-Carboxyfluorescein, succinimidyl ester mixed isomers) is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5. The amide linkage from the coupling reaction is much more stable than the thiourea linkage formed from the coupling of an amine and an isothiocyanate.
Supplier:
Adipogen
Description:
6-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside the cells, 6-CFDA is hydrolyzed by intracellular esterases to 6-carboxyfluorescein. Reagent for continuously determining the intracellular pH in bacteria by fluorometry. Labeling human intervertebral disk cells in vitro for fluorescence microscopy.
Supplier:
Adipogen
Description:
5-CFDA is membrane-permeant and can be loaded into cells via incubation. Once inside the cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein. It has been used for labeling human intervertebral disk cells in vitro for fluorescence microscopy. Continuous determination of the intracellular pH in bacteria by fluorometry.
Supplier:
Adipogen
Description:
Popular green fluorescent cell-permeable dye. Covalently couples via its succinimidyl group to intracellular molecules. The amine-reactive succinimidyl ester of 5-FAM acid is a common fluorescent derivatization reagent for covalently labeling peptides, proteins and nucleotides. Due to this covalent coupling reaction it can be retained within cells for extremely long periods and due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. Yields carboxyamides which are more resistant to hydrolysis than fluorescein conjugates prepared with FITC. Better retained in cells than fluorescein. Used in fluorescent small molecule preparations. Spectral data: lambdaex 494 nm| lambdaem 520 nm (Buffer pH 9.0).
Supplier:
AAT BIOQUEST INC
Description:
FAM-YVAD-FMK, i.e., 5-carboxyfluorescein-Tyr-Val-Ala-Asp-fluoromethylketone, binds irreversibly to active caspase 1 in stimulated cells.
Supplier:
Adipogen
Description:
Popular green fluorescent cell-permeable dye. Covalently couples via its succinimidyl group to intracellular molecules. The amine-reactive succinimidyl ester of 6-FAM acid is a common fluorescent derivatization reagent for covalently labeling peptides, proteins and nucleotides. Due to this covalent coupling reaction it can be retained within cells for extremely long periods and due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. Yields carboxyamides which are more resistant to hydrolysis than fluorescein conjugates prepared with FITC. Better retained in cells than fluorescein. Spectral data: lambdaex 496 nm| lambdaem 516 nm (Buffer pH 9.0).
Catalog Number:
(77979-474)
Supplier:
LGC STANDARDS
Description:
5-Carboxyfluorescein-N-hydroxysuccinimide Ester, TRC, LGC Standards
Catalog Number:
(103007-616)
Supplier:
Anaspec Inc
Description:
PMDM6-F is a fluorescent-labeled probe for MDM2-binding assay.
Sequence: 5-FAM-(β-A)-(β-A)-FM-Aib-pY-(6-Cl-DL-Trp)-E-Ac3c-LN-NH2 MW: 1784.3Da % peak area by HPLC:95 Storage condition:-20° C
Catalog Number:
(89139-252)
Supplier:
Biotium
Description:
5-(and-6)-carboxy-2′,7′-dichlorofluorescein is similar to carboxyfluorescein (51013), but has lower pKa. It is useful for detecting pH in more acidic environment.
Supplier:
Anaspec Inc
Description:
CREBtide is a synthetic substrate for PKA (Km = 3.9 µM). This peptide is based on the phosphorylation sequence in δ-CREB (cAMP response element binding protein).
Sequence:5-FAM-KRREILSRRPSYR MW:2075.3 Da % peak area by HPLC:95 Storage condition:-20° C
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 is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the
is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
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