Amberlite®+IRA-900+(Cl)
Supplier:
THERMO FISHER SCIENTIFIC CHEMICALS
Description:
Amberlite® IRA-900 (Cl) ion-exchange resin
Supplier:
MP Biomedicals
Description:
Chloride form quaternary ammonium functionality.Strongly basic, macroreticular resin of moderately high porosity with benzyltrialkylammonium functionality.
Supplier:
THERMO FISHER SCIENTIFIC CHEMICALS
Description:
Amberlite® IRA-410 (Cl) ion-exchange resin
Supplier:
THERMO FISHER SCIENTIFIC CHEMICALS
Description:
Amberlite® IRA-402 (Cl) ion-exchange resin
Supplier:
Thermo Scientific Chemicals
Description:
Styrene-DVB; Strongly basic anion exchange resin.
Supplier:
Thermo Scientific Chemicals
Description:
Acrylic-DVB; Weakly basic gel type resin
Supplier:
Thermo Scientific Chemicals
Description:
Amberlite|r IRA-96, ion exchange resin
Supplier:
Thermo Scientific Chemicals
Description:
Styrene-DVB; Strongly acidic macroreticular cation exchange resin
Supplier:
MP Biomedicals
Description:
Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose; used as a diuretic; enhances the action of sulfonamides; an antiseptic. Urea in solution is in equilibrium with ammonium cyanate. The form that reacts with protein amino groups is isocyanic acid. Urea in the presence of heat and protein leads to carbamylation of the proteins. Carbamylation by isocyanic acid interferes with protein characterization because isocyanic acid reacts with the amino terminus of proteins, preventing N-terminal sequencing. Isocyanic acid also reacts with side chains of lysine and arginine residues resulting in a protein that is unsuitable for many enzymatic digests. In addition, carbamylation often leads to confusing results from peptides having unexpected retention times and masses. When performing enzymatic protein digests it is important to remove urea first. Even though some enzymes will tolerate small amounts of urea, the elevated temperature used for most reactions will lead to carbamylation during the course of the digest. The urea can be removed prior to digestion by fast reversed phase chromatography, spin columns, or dialysis. Dissolve urea in deionized water to the desired concentration.For every 10 ml of solution, add 1 g of Amberlite® IRA-910.Stir for one hour at room temperature
Supplier:
TCI America
Description:
CAS Number: 101-14-4
MDL Number: MFCD00047829 Molecular Formula: C13H12Cl2N2 Molecular Weight: 267.15 Purity/Analysis Method: >90.0% (HPLC) Form: Crystal Melting point (°C): 108 Flash Point (°C): 110
Supplier:
TCI America
Description:
CAS Number: 69235-50-3
MDL Number: MFCD00069039 Purity/Analysis Method: >90.0% (HPLC) Form: Crystal
Catalog Number:
(TCP0598-025G)
Supplier:
TCI America
Description:
CAS Number: 3521-06-0
MDL Number: MFCD00050260 Molecular Formula: C23H24Cl2N2 Molecular Weight: 399.36 Purity/Analysis Method: >90.0% (HPLC) Form: Crystal Lambda max.: 630 nm (H2O)
Supplier:
TCI America
Description:
CAS Number: 3511-19-1
MDL Number: MFCD00059736 Molecular Formula: C4H6Cl2O Molecular Weight: 140.99 Purity/Analysis Method: >90.0% (GC) Form: Clear Liquid Boiling point (°C): 63 Specific Gravity (20/20): 1.34
Catalog Number:
(TCM1156-005G)
Supplier:
TCI America
Description:
CAS Number: 37632-18-1
MDL Number: MFCD00123296 Molecular Formula: C7H7Cl2O2P Molecular Weight: 225.00 Purity/Analysis Method: >90.0% (GC) Form: Clear Liquid Specific Gravity (20/20): 1.42
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