Electrophoresis+Stains
Supplier:
Argos Technologies
Description:
These heavy-duty 1000W ovens can be used for heating media, electrophoresis applications, staining preparations, or drying samples for moisture analysis, and are an effective alternative to hot plates or convection ovens
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Supplier:
Thermo Scientific Chemicals
Description:
DNA stain useful as tracking dye in acid buffers for electrophoresis. Binds selectively to AT-rich regions of native DNA.
Supplier:
Corning
Description:
These dishes made of PYREX® borosilicate glass will withstand hot air or steam sterilization and are useful for drying, staining or developing trays.
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Supplier:
Thermo Scientific Chemicals
Description:
Sodium acetate buffer solution pH, 5.2 is been widely used in for precipitation of nucleic acids and preparation of gel stains for protein gel electrophoresis. Sodium acetate buffer solution is suitable for precipitation of DNA, elution of proteins and for use in molecular biology applications such as Chip-on-Chip assay and in vitro enzyme inhibition assay.
Supplier:
GE Healthcare - Life Sciences
Description:
IPG buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobilineâ„¢ DryStrip gels for isoelectric focusing, run as the first dimension of 2-D electrophoresis or as a separate application. Using Immobiline DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting low abundance proteins.
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Catalog Number:
(10098-684)
Supplier:
Biotium
Description:
GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes are DNA gel loading buffers designed to replace the highly toxic ethidium bromide for one-step fluorescent staining and gel loading of DNA.
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Catalog Number:
(PAH5041)
Supplier:
Promega Corporation
Description:
Ethidium Bromide Solution, Molecular Grade (10mg/ml), is a fluorescent dye suitable for staining nucleic acids after electrophoresis or in cesium chloride gradients. The solution can be used to detect both double-stranded and single-stranded DNA.
Catalog Number:
(95044-738)
Supplier:
GE Healthcare - Life Sciences
Description:
The key to reproducible electrophoresis results is temperature control. Gels cooled on their two faces produce slanted bands appearing as broad or double bands in stained gels. SE 600 Ruby ensures uniform heat in gels because, the gels are immersed in the lower buffer for uniform heat transfer and a standard magnetic stirrer circulates buffer around the vertically suspended heat exchanger to maintain homogeneous lower buffer temperature. The ergonomic SE 600 Ruby provides two built-in side handles, which facilitates carrying and handling. Run up to four gels (up to 112 samples) in a single run. Divider plates convert single-gel sandwiches into two-gel club sandwiches, which seal and fit into the casting stand like single gels. If gels longer than 16-cm are required, Cytiva offers the SE 660, which runs gels up to 24 cm. Except for gel lengt, the SE 660 has identical features to those of SE 600 Ruby.
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Supplier:
Thermo Scientific Chemicals
Description:
Protein staining following gel electrophoresis
Supplier:
Adipogen
Description:
Activated ester of ruthenium complex for acylation of amino acid side chain amines. This label is perfectly suitable for 1D- or 2D-protein gel staining. A simple pre-electrophoresis procedure provides a sensitivity better than SYPRO Ruby and a similar dynamic range. In contrast to SYPRO Ruby, this staining exhibits a logarithmic dependency on the amount of protein.
Catalog Number:
(IC802511)
Supplier:
MP Biomedicals
Description:
A 10mg/mL easy-to-use solution of ethidium bromide in specially filtered, deionized water which is excellent for nucleic acid electrophoresis and purification applications, eliminates the dust hazard associated with powdered ethidium bromide and saves time spent on weighing and mixing. Used in the fluorometric detection of double stranded nucleic acids in gel electrophoresis. Also acts as an RNA polymerase inhibitor, and in separation of high molecular weight DNA's. It confers a deep red stain to DNA by acting as an intercalating agent between the starch bases. Subsequent fluorescence techniques enable the extraction and characterization of DNA bands.
Catalog Number:
(EM-3340)
Supplier:
MilliporeSigma
Description:
Powder. Use for post-electrophoresis staining of proteins in applications requiring a high degree of sensitivity and/or solubility.
Catalog Number:
(470136-468)
Supplier:
Edvotek
Description:
For centuries, humans have used selective breeding and conventional hybridization to produce desirable qualities and to increase crop yields. Today, scientists use genetic engineering to directly manipulate the DNA, quickly producing these desirable traits. In this experiment, students will use agarose gel electrophoresis to explore the molecular methods used by scientists to identify genetically modified organisms. No thermal cycler is required. Students are also encouraged to explore the controversy surrounding the use of genetically modified organisms.
Catalog Number:
(95043-424)
Supplier:
G-Biosciences
Description:
G-Biosciences' Coomassie Brilliant Blue (CBB) G-250 and R-250 Stain are based on a colloidal Coomassie stain. CBB G-250 and R-250 stain proteins with high band visibility. The staining of gels with CBB G-250 and R-250 allows the examination of protein bands even during the staining process.
Catalog Number:
(95043-420)
Supplier:
G-Biosciences
Description:
G-Biosciences' Coomassie Brilliant Blue (CBB) G-250 and R-250 Stain are based on a colloidal Coomassie stain. CBB G-250 and R-250 stain proteins with high band visibility. The staining of gels with CBB G-250 and R-250 allows the examination of protein bands even during the staining process.
Supplier:
MP Biomedicals
Description:
Agarose is a natural polysaccharide isolated from agar or agar-bearing marine algae. Its physical and chemical properties are particularly suited for use as a gel medium for diffusion and electrokinetic movement of biomolecules. Agarose is essentially biologically inert and forms relatively clear, transparent gels. Agarose based gels offer several advantages: High gel strength permits use of low concentrations. The macroporous nature of the gels allows rapid diffusion of high molecular weight molecules. Agarose is Non-toxic. Agarose gels are thermally reversible facilitating sample recovery. Staining and destaining may be done rapidly with minimal background. Because agarose forms macroporous gels that are electrically nonionic, it is an excellent medium for Isoelectric Focusing (IEF). A selection of different formulated agaroses with specific qualities for unique applications are available from MP.
Multipurpose Agarose is a versatile high gel strength agarose, especially designed for a wide range of molecular biology techniques. It is used for resolution of fragments over a large size range from 200 bp up to 50 kb by conventional electrophoresis. It is suitable for use in pulsed field gel electrophoresis and for blotting assays. High gel strength agarose, especially designed for a wide range DNA. Versatile – separate fragments from 200 bp up to 50 kb by conventional electrophoresis. Concentrations between 0.4% and 6%. Multipurpose Agarose features the highest gel strength available, allowing 0.4 - 0.5% agarose gels. Significantly decreases electrophoresis times. Improves separation of very large fragments. Dissolves easily in a microwave.
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