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Description:
BES is a zwitterionic buffer that is used in biochemistry and molecular biology research in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies.
Description:
( [N,N-bis-(2-Hydroxyethyl)-2-aminoethanesulfonic acid]). White solid. BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable.
Description:
One of the best general-purpose buffers for biological research. Excellent for tissue culture, oxidative phosphorylation, protein synthesis with cell-free bacterial systems, photophosphorylation, and CO<sub>2</sub> fixation.
Description:
HEPES, Biological Buffer is as it's name suggests, a buffering agent. It's a zwitterionic organic chemical that is one of the '20 Good's buffers.' Because it's better at maintaining physiological pH despite changes in carbon dioxide, it is widely used in cell culture. It has a decrease of water dissociation with falling temperature, this is especially relevant when working at low temperatures as it maintains enzyme function and structure. That said, it is important when working with HEPES for that particular purpose to keep the HEPES-containing solution in darkness as much as possible
Description:
A zwitterionic N-substituted aminosulfonic acid buffer. HEPES has its maximum buffering capacity very near the pH of mammalian tissue fluids (pKa of 7.31 at 37°C). Exhibits no metal binding and has a small temperature dependent ΔpKa of -0.014/°C.