Catalog Number:
(89165-938)
Supplier:
Enzo Life Sciences
Description:
Calcium dye
Catalog Number:
(80051-690)
Catalog Number:
(89139-184)
Supplier:
Biotium
Description:
Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, the mild detergent Pluronic F-127 (catalog no. 59004) is often used as a dispersing agent to facilitate loading. Fluo-3 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to fluo-3 by endogenous esterases once the dye is inside the cells. An important application of Fluo-3 AM ester is its use in high throughput drug screening.
Catalog Number:
(76483-132)
Supplier:
AAT BIOQUEST INC
Description:
Calcium measurement is critical for numerous biological investigations.
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Catalog Number:
(76483-138)
Supplier:
AAT BIOQUEST INC
Description:
Calcium measurement is critical for numerous biological investigations.
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Supplier:
Biotium
Description:
Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Fluo-3 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to Fluo-3 by endogenous esterases once the dye is inside the cells. An important application of Fluo-3 AM ester is its use in high throughput drug screening.
Supplier:
AAT BIOQUEST INC
Description:
Calcium measurement is critical for numerous biological investigations.
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Supplier:
Adipogen
Description:
Ca2+-indicator fluo-3 for use with visible-light excitation sources in flow cytometry and confocal laser-scanning microscopy. Since about 1996, fluo-3 has also been extensively used in cell-based high-throughput screening assays for drug discovery. Fluo-3 AM is essentially nonfluorescent until it is hydrolyzed intracellularly and/or in the presence of Ca2+. The green-fluorescent emission (~525 nm) of Ca2+-bound fluo-3 is conventionally detected using optical filter sets designed for fluorescein (FITC). As prepared by Minta, Kao and Tsien, fluo-3 was originally reported to undergo an ~40-fold increase in fluorescence upon binding Ca2+.
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