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Description:
Potassium acetate is used as a buffer in molecular biology research, notably in the isolation of DNA. Potassium acetate may be utilized in the isolation of RNA intermediates and products from in vitro splicing reactions performed with HeLa cell nuclear extract. Potassium acetate has been used to study the interactions between the TATA box binding protein from Pyrococcus woesei and an oligonucleotide with a specific binding site. The use of potassium acetate in the analysis of oligogalacturonic acids by highperformance anion-exchange chromatography has been described.
Description:
Ethyl Acetate is an organic solvent with the structure CH<sub>3</sub>-COO-CH<sub>2</sub>-CH<sub>3</sub> (C<sub>4</sub>H<sub>8</sub>O<sub>2</sub>). This colorless liquid has a characteristic sweet odor. In the laboratory, mixtures containing ethyl acetate are used in column chromatography and extractions. Ethyl acetate does not work well as a reaction solvent because it is prone to hydrolysis and transesterification. Ethyl acetate is volatile at room temperature and has a boiling point of 77 °C. Suitable for GC and Pesticide Residue Analysis.