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E. Becker & Co. GmbH
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E. Becker & Co. GmbH
Catalog Number:
(IC091676549)
Supplier:
MP Biomedicals
Description:
MP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media.
Catalog Number:
(75877-480)
Supplier:
MP Biomedicals
Description:
Fluorescein-conjugated Goat IgG fraction to Rabbit IgG is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to rabbit IgG (whole molecule) and buffer salts.
IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Anti-Rabbit IgG (whole molecule)-FITC antibody reacts with rabbit serum and rabbit IgG. Fluorescein-conjugated Goat IgG fraction to Rabbit IgG is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals). Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized. The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized by equivalence-point precipitation. The IgG fraction is tested for purity and specificity at 40 mg/mL using immunoelectrophoresis. The product is mostly goat IgG; no trace of albumin is detected.
Catalog Number:
(IC0856007)
Supplier:
MP Biomedicals
Description:
Product is the lyophilized powder of goat antiserum to swine serum and buffer salts.
Catalog Number:
(IC0855111)
Supplier:
MP Biomedicals
Description:
Product is liquid goat antiserum to human alpha-1-antitrypsin and buffer salts.
The antiserum is prepared by hyperimmunizing goats with highly purified human alpha-1-antitrypsin; antiserum is delipidated and made specific by solid-phase absorption. The resulting antiserum is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed.
Catalog Number:
(IC116850200)
Supplier:
MP Biomedicals
Description:
BioPulverizerâ„¢ System II contains 10x2 mL tubes of each of our specialized Lysing Matrices.
Catalog Number:
(IC091682049)
Supplier:
MP Biomedicals
Description:
Sodium pyruvate is used by cells as an easily accessible carbohydrate source. Additionally, it is involved with amino acid metabolism and initiates the Kreb′s cycle. The 100 mM solution should be diluted 1:100 for most cell culture. The use of sodium pyruvate in Wallen fermentation medium to enhance the conversion of oleic acid to 10-ketostearic acid by Bacillus sphaericus has been described. A protocol that uses sodium pyruvate to establish stably transfected human B cell lines has been published. It improves coliform recovery when present in culture medium.
Supplier:
MP Biomedicals
Description:
~28 IU/mg, Powder
Catalog Number:
(76522-168)
Supplier:
MP Biomedicals
Description:
Adapter for FastPrep-96 instruments.
Supplier:
MP Biomedicals
Description:
Sterile 80% Glycerol solution offered is less viscous and more convenient to pipette than 100% glycerol.
Catalog Number:
(IC092750016)
Supplier:
MP Biomedicals
Description:
Pluronic F-68® is a difunctional block copolymer surfactant terminating in primary hydroxyl groups. A nonionic surfactant that is 100% active and relatively non-toxic.
Supplier:
MP Biomedicals
Description:
PBS 10X is frequently used as a wash and dilution buffer.
Catalog Number:
(76202-438)
Supplier:
MP Biomedicals
Description:
LSMâ„¢ is a sterile-filtered solution of a sucrose polymer and diatrizoate salts.
Catalog Number:
(75877-482)
Supplier:
MP Biomedicals
Description:
Peroxidase-conjugated goat IgG fraction to rabbit IgG (whole molecule) is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to rabbit IgG (whole molecule) and buffer salts. Anti-Human IgG is developed in goat using IgG from pooled normal human serum as the immunogen. Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other goat serum proteins.
Specificity for human IgG is determined by immunoelectrophoresis (IEP) versus purified human IgA, IgG, IgM, Bence Jones kappa, and Bence Jones lambda myeloma proteins. Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum result in single arcs of precipitation in the gamma region. Peroxidase-conjugated goat IgG fraction to rabbit IgG (whole molecule) product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals. Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized. The total protein is measured using the Biuret procedure with bovine albumin as the standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with rabbit IgG. The product is tested for purity and specificity at final concentration by immunoelectrophoresis. The antibody is predominantly goat IgG; no trace of albumin is detected.
Catalog Number:
(IC780331)
Supplier:
MP Biomedicals
Description:
Form: Lyophilized powder.
Reconstitution: Reconstitute with 0.01M sodium phosphate-0.15M sodium chloride, pH 7.2-7.4. Activity: Shows agglutination when tested with human red blood cells.
Catalog Number:
(IC15883701)
Supplier:
MP Biomedicals
Description:
Typical working concentration for 100 mL of inhibitor cocktail: AEBSF Hydrochloride:50-400 mg/mL; EDTA disodium dihydrate:0.2-25 mg/mL; Leupeptin Hemisulfate: 0.5-1 mg/mL; Pepstatin A: 0.5-1 mg/mL
Catalog Number:
(IC0219378125)
Supplier:
MP Biomedicals
Description:
Sulforaphane is an anti-cancer, anti-microbial and anti-diabetic compound found in cruciferous vegetables.
Selective inducer of phase II detoxification enzymes with anticarcinogenic properties. Organosulfur compound found in cruciferous vegetables, including broccoli. It induces the production of detoxifying enzymes such as quinone reductase and glutathione S-transferase that cause xenobiotic transformation. Sulforaphane also increases the transcription of tumor suppressor proteins and inhibits histone deacetylases. It modulates inflammatory responses by suppressing the LPS-mediated expression of iNOS, COX-2, IL-1β and TNF-α.
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 is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the
is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
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