Paul Schöndorf Metallwaren GmbH
Supplier:
Invitrogen
Description:
SuperSignal™ ELISA Pico chemiluminescent substrate is optimized to generate an intense light signal and provide exceptional performance in luminometer based assays.
Catalog Number:
(PI88434)
Supplier:
Invitrogen
Description:
Thermo Scientific Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits.
Supplier:
Invitrogen
Description:
Thermo Scientific Pierce EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.
Supplier:
Invitrogen
Description:
Thermo Scientific Pierce Anti-c-Myc Magnetic Beads are affinity particles for immunoprecipitation of recombinant c-Myc-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.
Supplier:
Invitrogen
Description:
Simplifies dialysis of sample volumes between 15 ml and 100 ml.
Catalog Number:
(PI21629)
Supplier:
Invitrogen
Description:
Use to develop in-house non-radioactive assay systems.
Catalog Number:
(PI21516)
Supplier:
Invitrogen
Description:
The Pierce™ GST Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with GST-tagged fusion proteins.
Catalog Number:
(PI29139)
Supplier:
Invitrogen
Description:
Biotinylated horse radish peroxidase is used as control or signal amplification in IHC to amplify the signal of biotinylated primary antibodies using the avidin-biotin complex staining method.
Catalog Number:
(PI34035)
Supplier:
Invitrogen
Description:
NBT (nitro-blue tetrazolium chloride) yields a blue-purple, water-insoluble product upon reduction of the tetrazolium salt
Catalog Number:
(PI88869)
Supplier:
Invitrogen
Description:
Thermo Scientific T7 Cell-Free Expression Vectors (pT7CFE1) are cloning plasmids optimized to use with the 1-Step Human <i>in vitro</i> Protein Expression System for in vitro translation (IVT) of tagged fusion proteins.
Supplier:
Invitrogen
Description:
Thermo Scientific Pierce Anti-c-Myc Agarose is a high-affinity immunoprecipitation resin ideal for purification of recombinant c-Myc-tagged proteins expressed in human <i>in vitro</i> expression systems and bacterial and mammalian cell lysates.
Catalog Number:
(PI22327)
Supplier:
Invitrogen
Description:
Thermo Scientific Pierce Sulfo-SIAB a water-soluble, mid-length (10.6 angstrom) crosslinker for amine-to-sulfhydryl conjugation via sulfo-N-hydroxysuccinimide (sulfo-NHS) ester and iodoacetyl reactive groups.
Catalog Number:
(PI16189)
Supplier:
Invitrogen
Description:
The Thermo Scientific Pierce Luciferase Cell Lysis Buffer effectively lyses adherent cells or pelleted suspension cells for analysis of promoter expression in luciferase assays.
Catalog Number:
(PI26180)
Supplier:
Invitrogen
Description:
Pierce™ HA-Tag IP/Co-IP kit provides the affinity resin, positive control and other reagents necessary to perform immunoprecipitation (IP) or co-immunoprecipitation (Co-IP) reactions with an HA-tagged bait protein.
Supplier:
Invitrogen
Description:
Thermo Scientific Pierce Protein A/G Magnetic Beads are high-performance affinity particles for antibody purification and immunoprecipitation methods using manual or robotic magnetic separators.
Catalog Number:
(PI78433)
Supplier:
Invitrogen
Description:
Bestatin Protease Inhibitor reversably inhibits amino peptidases.
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