Paul Schöndorf Metallwaren GmbH
Supplier:
Invitrogen
Description:
Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions.
Catalog Number:
(PI22305)
Supplier:
Invitrogen
Description:
Thermo Scientific Pierce MPBH is a long, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).
Catalog Number:
(PI62237)
Supplier:
Invitrogen
Description:
Thermo Scientific myImageAnalysis Software is a full-featured, multifunction computer program to analyze and edit digital images of electrophoresis gels and blots that were acquired using gel documentation imagers or scanners.
Catalog Number:
(PI21277)
Supplier:
Invitrogen
Description:
The Pierce™ His Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with His-tagged fusion proteins.
Catalog Number:
(PI62280)
Supplier:
Invitrogen
Description:
DyLight™ fluor-free acids are non-activated forms of selected DyLight™ fluorescent dyes for use as controls and calibration factors in fluorescence imaging applications
Catalog Number:
(PI34070)
Supplier:
Invitrogen
Description:
The 1-Step™ NBT and BCIP substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.
Catalog Number:
(PI84747)
Supplier:
Invitrogen
Description:
The Western Blot Roller is a simple, reusable tool for gently and easily removing trapped air bubbles when assembling transfer stacks in semi-dry or wet-tank transfer cassettes.
Catalog Number:
(PI87759)
Catalog Number:
(PI24612)
Supplier:
Invitrogen
Description:
The Pierce Silver Stain Kit is a rapid, ultra-sensitive, and versatile silver stain system for protein detection in polyacrylamide gels, yielding consistent and reliable results. It is a metallic silver (Ag) protein stain that yields a remarkably clear and uniform gel background. In standard mini gels, proteins are detectable at greater than 0.25 ng per band or spot. The protocol has been optimized for flexibility by allowing short or overnight gel fixation and staining steps without affecting staining performance (sensitivity or clarity). A short (one-minute) sensitization step, performed after gel fixation, yields results that are free of the characteristically dark or blotchy backgrounds often seen with homemade or other commercially available silver stains. This feature is beneficial when performing densitometric analysis of silver stained gels.
Catalog Number:
(PI44892)
Supplier:
Invitrogen
Description:
Group: AminoLink Kit and AminoLink Coupling Gel. Links with primary amines on proteins, peptides, antigens or antibodies. Features/Benefits:. AminoLink Coupling Gel is a 4% cross-linked beaded agarose support, activated to form aldehyde functional groups.
Supplier:
Invitrogen
Description:
Blocker BSA in PBS or TBS are ready-to-use, 1X PBS or TBS solutions of 10X (10%) bovine serum albumin protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.
Catalog Number:
(PI89972)
Supplier:
Invitrogen
Description:
Thermo Scientific Melon Gel Purification Buffer, formulation: proprietary buffer dry-blend, sufficient for preparing 200 mL of 100X buffer.
Supplier:
Invitrogen
Description:
Pierce™ Streptavidin Plus UltraLink™ resin consists of purified recombinant streptavidin that has been covalently immobilized onto a beaded acrylamide support
Supplier:
Invitrogen
Description:
Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.
Catalog Number:
(PI25108)
Supplier:
Invitrogen
Description:
Thermo Scientific Pierce Triethylamine (TEA) is a high-purity solvent that is used in certain HPLC protocols, such as weak anion exchange, to resolve certain tryptic peptides on a reverse-phase column.
Catalog Number:
(PI77110)
Supplier:
Invitrogen
Description:
Thermo Scientific Imject BSA in PBS is purified bovine serum albumin carrier protein that enable simple preparation of effective immunogens with amine- or carboxyl-peptide antigens.
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