Potters-Ballotini GmbH
Supplier:
MP Biomedicals
Description:
Tris has been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). Tris does not precipitate calcium salts and is of value in maintaining solubility of manganese salts. It can be used for the direct standardization of a strong acid solution; the equivalence point can be determined either potentiometrically or by use of a suitable indicator such as 3-(4-Dimethylamino-1-naphthylazo)-4-methoxybenzenesulfonic acid. It is an auxiliary material in pharmaceutical science.
Store at Room Temperature (15-30 °C). Store dessicated.
Catalog Number:
(PAG1471)
Supplier:
Promega Corporation
Description:
Human Male Genomic DNA is purified and stored in 10mM Tris-HCl (pH 8.0), 1mM EDTA >90% of the DNA is longer than 50kb in size as measured by pulsed-field gel electrophoresis. The DNA is suitable for Southern blot hybridizations, genomic analysis (including PCR) and genomic library construction. Human Genomic DNA comes from multiple anonymous donors.
Supplier:
G-Biosciences
Description:
G-Biosciences' GenLysateâ„¢ total protein lysates are ready to use for SDS-PAGE and Western blotting purposes.
Supplier:
G-Biosciences
Description:
G-Biosciences' FOCUSâ„¢ Protein Alkylation Kit contains an ultra pure, proteomic grade iodoacetamide reagent for blocking thiols by alkylation in order to prevent the reoxidation of proteins
Catalog Number:
(PI37527)
Supplier:
Invitrogen
Description:
This Fish Serum Blocking Buffer is Steelhead Salmon Serum in PBS that is especially useful as a blocking agent in IHC and other detection methods involving mammalian samples.
Catalog Number:
(103853-666)
Supplier:
BioVendor
Description:
EndoTrap® HD removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies, vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rate.
The EndoTrap® HD resin is especially designed to meet the needs of pharma and biotech industry for the efficient removal of endotoxins in biomanufacturing processes as well as any application that requires highest endotoxin removal rates. EndoTrap® HD fulfils all requirements for industrial applications, and it can easily be integrated in/at early or late-stage purification processes. Moreover, the High-Definition Matrix is also available for small-scale purification as pre-filled columns. Endotoxin removal with EndoTrap® HD works effectively in the pH range of 4-9 and NaCl concentrations of 50-600 mM. Buffers such as HEPES, TRIS, MOPS, MES, and PIPES are recommended. Citrate buffers and chelators of divalent cations (like EDTA) have to be avoided
Supplier:
MP Biomedicals
Description:
Boric acid is used for weatherproofing wood and fireproofing fabrics; as a preservative; manufacturing of cements, crockery, porcelain, enamels, glass, borates, leather, carpets, hats, soaps, artificial gems; in nickeling baths; cosmetics; printing and dyeing, painting; photography; for impregnating wicks; electric condensers; hardening steel. It is also used as an insecticide for cockroaches and black carpet beetles. It is used In the research labs , astringent, antiseptic, antibacterial and antifungal agent. It is used in buffers and for calibration of polyacrylamide gel columns for the separation of oligonucleotides by capillary electrophoresis.
Catalog Number:
(103737-104)
Supplier:
BioVendor
Description:
EndoTrap® HD removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies, vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rate.
The EndoTrap® HD resin is especially designed to meet the needs of pharma and biotech industry for the efficient removal of endotoxins in biomanufacturing processes as well as any application that requires highest endotoxin removal rates. EndoTrap® HD fulfils all requirements for industrial applications, and it can easily be integrated in/at early or late-stage purification processes. Moreover, the High-Definition Matrix is also available for small-scale purification as pre-filled columns. Endotoxin removal with EndoTrap® HD works effectively in the pH range of 4-9 and NaCl concentrations of 50-600 mM. Buffers such as HEPES, TRIS, MOPS, MES, and PIPES are recommended. Citrate buffers and chelators of divalent cations (like EDTA) have to be avoided
Catalog Number:
(IB07062)
Supplier:
IBI Scientific
Description:
SDS is an ionic detergent used to denature proteins in hybridization, nucleic purification, and electrophoresis buffer systems. SDS is also used to dissociate nucleic acid protein complexes in DNA extraction protocols, to disrupt cell membranes and to prepare prehybridization and/or hybridization solutions.
Supplier:
MP Biomedicals
Description:
β-Mercaptoethanol can act as an enzyme reactivator in systems necessitating reduction for activation, and has been commonly used to reduce disulfide bonds in order to separate protein subunits for use in electrophoresis.
2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.
Catalog Number:
(470136-468)
Supplier:
Edvotek
Description:
For centuries, humans have used selective breeding and conventional hybridization to produce desirable qualities and to increase crop yields. Today, scientists use genetic engineering to directly manipulate the DNA, quickly producing these desirable traits. In this experiment, students will use agarose gel electrophoresis to explore the molecular methods used by scientists to identify genetically modified organisms. No thermal cycler is required. Students are also encouraged to explore the controversy surrounding the use of genetically modified organisms.
Catalog Number:
(PAG3091)
Supplier:
Promega Corporation
Description:
Mouse Genomic DNA is purified and stored in 10mM Tris-HCl (pH 8.0), 1mM EDTA; >90% of the DNA is longer than 50kb in size as measured by pulsed-field gel electrophoresis. The DNA is suitable for Southern blot hybridizations, genomic analysis (including PCR), and genomic library construction. Mouse Genomic DNA is isolated from whole blood from disease-free mice.
Supplier:
MP Biomedicals
Description:
Ethylenediamine tetraacetic acid (EDTA) is a calcium chelator used to eliminate inhibition of enzyme catalyzed reactions due to traces of heavy metals.
Catalog Number:
(PAG3041)
Supplier:
Promega Corporation
Description:
Human mixed male and female Genomic DNA is purified and stored in 10mM Tris-HCl (pH 8.0), 1mM EDTA >90% of the DNA is longer than 50kb in size as measured by pulsed-field gel electrophoresis. The DNA is suitable for Southern blot hybridizations, genomic analysis (including PCR) and genomic library construction. Human Genomic DNA comes from multiple anonymous donors.
Catalog Number:
(IC04814326)
Supplier:
MP Biomedicals
Description:
Acrylamide is a monomer used in a variety of synthetic processes to form polymers and copolymers. It polymerizes in the presence of free radicals in aqueous solutions. It is usually used to prepare polyacrylamide gels for electrophoresis separation of biomolecules. Common reaction initiators are riboflavin and ammonium persulfate; varying the ratio of acrylamide to crosslinking agent permits the formation of a gel with predictable average pore size and texture. Acrylamide tends to hydrolyze under acidic or basic conditions to form acrylic acid. Polyacrylamide plastics have numerous commercial applications, due in part to the tendency of polyacrylamide to adsorb many times its own mass in water. Acrylic acid and any ionic impurities in the acrylamide can have significant effects on the performance of the acrylamide gel formed, since the voltage across the gel is affected by the ionic charge of the gel and usage buffers.
Store at Room Temperature (15-30 °C), Dessicated.
Catalog Number:
(12001-392)
Supplier:
Lonza
Description:
AccuGENEâ„¢ Molecular Biology Buffers are ready-to-use solutions ideal for a wide range of molecular biology applications.
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