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Description:
Immunohistochemistry (IHC) is the localization of antigens in tissue sections/cells using antibodies as specific reagents. Visualization of antibody signal is often significantly improved using the biotin-avidin amplification system. This system, however, adds significant time to experimental protocols and results in higher background due to the presence of endogenous biotin in tissue.
GeneTex’s One-Step anti-mouse and rat IgG (H+L) uses the power of next-generation Polymer technology to provide dramatic signal amplification without the need for biotin. This reagent has been shown to provide increased sensitivity and detection, while reducing the incubation time and required quantity of primary antibody. This reagent can be used to enhance staining of membranes, cytoplasmic and nuclear antigens. It provides the user with a rapid and easy to use IHC detection system. Features and Benefits
‧ Lower Amplification without biotin avoids background from endogenous biotin ‧ Higher sensitivity than standard IHC detection ‧ Faster: Staining time reduced to 15 minutes! ‧ Compatible with either manual or automated systems ‧ Better results on difficult-to-work antibodies and nuclear staining ‧ Save cost due to further dilution of primary antibody
Description:
UltraPlex 1-Step ToughMix is a ready-to-use, 4X concentrated master mix 1-step reverse transcription and real-time quantitative PCR (RT-qPCR) of RNA templates using probe-based detection methods. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between steps. Optimized to deliver highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or highly multiplexed RNA detection assays, this reagent chemistry is optimized to deliver maximum assay sensitivity, precision and reproducibility with miniaturized reaction volumes and either conventional or accelerated thermal cycling protocols. UltraPlex 1-Step ToughMix contains all required components for RT-qPCR except RNA template and probe and is compatible with all dual-labeled probe chemistries.
Description:
Q5U Hot Start high-fidelity DNA Polymerase is a modified version of Q5 high-fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3' to 5' exonuclease activity, and is fused to a processivity-enhancing Sso7d domain.
Description:
iFluor® 647 PSA kit is a much superior replacement for Alexa Fluor 647 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
Description:
iFluor® 488 PSA kit is a much superior replacement for Alexa Fluor 488 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
Description:
qScript XLT One-Step RT-qPCR ToughMix is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5’-hydrolysis probes on real-time PCR systems that do not require a passive reference dye. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. qScript XLT One-Step RT-qPCR ToughMix contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.
Description:
Biotin derivative. Substrate of the horseradish peroxidase enzyme and used as a reagent to amplify immunohistochemical signals. It is based on the HRP-catalyzed deposition of tyramide conjugates (such as biotinyl-tyramide) on a solid phase. Subsequent reaction with streptavidin fluorophore results in the localization of the fluorophore at the site of tyramide deposition. This fluorescence-based tyramide signal amplification (TSA) has been widely used in immunohistochemistry, immunohistochemistry, immunoelectron microscopy, fluorescent in situ hybridization (FISH) and fluorescence ELISA. The TSA method has been reported to increase the detection sensitivity up to 100-fold as compared with conventional avidin–biotinylated enzyme complex procedures. It can be used together with both chromogenic and fluorescence visualization methods. It can be added to any other standard IHC protocol and reduces the use of other reagents; improves signal to noise by reducing the titer of other reagents in the assay protocol and enables multi-target detection in both IHC and (F)ISH applications.
Description:
i7™ high-fidelity DNA polymerase 2X master mix is a ready to use premix which contains high-fidelity DNA Polymerase, dNTPs, MgCl₂, PCR enhancers and stabilizers with optimized proprietary reaction buffer. i7™ high-fidelity DNA polymerase 2X master mix has the high-fidelity, sensitivity and processivity with an error rate ~2.8×102 fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes.
Description:
This SuperMix is a 2x concentrated, ready-to-use reaction cocktail for real-time quantitative PCR (qPCR) that contains all components, except primers, probes, and templates
Description:
Storage: Room Temperature, desiccate, store under nitrogen. Dimethyl Sulfoxide is a dipola, aprotic solvent. It has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination. Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly (A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols. To prepare sterile solutions use a teflon or nylon membrane to sterile-filter the DMSO - do not use a cellulose acetate membrane.
Description:
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Antibody to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
Description:
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Antibody to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
,10326-136EA
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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