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Paul Schöndorf Metallwaren GmbH
Supplier:
Invitrogen
Description:
The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.
Catalog Number:
(PI22297)
Supplier:
Invitrogen
Description:
Thermo Scientific Pierce BMPH is a short, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).
Supplier:
Invitrogen
Description:
Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications
PNPP (p-Nitrophenyl Phosphate, Disodium Sal) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution, and a complete kit containing substrate solution and diethanolamine buffer. Thermo Scientific Pierce PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm.
Catalog Number:
(PI35000)
Supplier:
Invitrogen
Description:
Thermo Scientific Pierce Peroxidase Suppressor is a stable, easy-to-use inhibitor of endogenous peroxidase activity from cells and tissues to reduce background signal in HRP-based immunohistochemistry applications.
Catalog Number:
(PI34065)
Supplier:
Invitrogen
Description:
Thermo Scientific Pierce™ Metal-Enhanced DAB Substrate Kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate.
Supplier:
Invitrogen
Description:
SuperSignal™ ELISA Pico chemiluminescent substrate is optimized to generate an intense light signal and provide exceptional performance in luminometer based assays.
Catalog Number:
(PI53104)
Supplier:
Invitrogen
Description:
Thermo Scientific Pierce Heptafluorobutyric Acid (HFBA), HPLC Grade is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.
Supplier:
Invitrogen
Description:
Thermo Scientific Pierce MS(PEG)<sub>n</sub> reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) and TMS(PEG)<sub>12</sub> is a three-branched, polyethylene glycol compound (3 x 12 PEG units). Both are activated as NHS esters for covalent pegylation of primary amines on proteins (eg. lysines) or assay surfaces.
Supplier:
Invitrogen
Description:
Thermo Scientific NeutrAvidin coated plates are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.
Catalog Number:
(PIN600)
Supplier:
Invitrogen
Description:
Stop Solution is 0.16M sulfuric acid for use with the ELISA substrate 3,3’,5,5’ - tetramethylbenzidine (TMB). In the presence of horseradish peroxidase (HRP) enzyme conjugates, TMB and peroxide react to produce a blue byproduct having maximum absorbance at 605nm. The color intensity is proportional to the amount of HRP activity, which in turn is related to the levels of target analyte in an optimized ELISA procedure. Addition of sulfuric acid stop solution changes the color to yellow (absorbance maximum at 450nm), stabilizing the color development to enable accurate measurement of the intensity at 450nm using a spectrophotometer or plate reader.
Catalog Number:
(PI22363)
Supplier:
Invitrogen
Description:
Thermo Scientific Pierce SMPH is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long spacer arm (14.2 angstroms).
Catalog Number:
(PI22339)
Supplier:
Invitrogen
Description:
Thermo Scientific Pierce SBAP is a short (6.2 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and bromoacetyl reactive groups.
Catalog Number:
(PI22327)
Supplier:
Invitrogen
Description:
Thermo Scientific Pierce Sulfo-SIAB a water-soluble, mid-length (10.6 angstrom) crosslinker for amine-to-sulfhydryl conjugation via sulfo-N-hydroxysuccinimide (sulfo-NHS) ester and iodoacetyl reactive groups.
Supplier:
Invitrogen
Description:
Pierce peptide retention time calibration mixture enables reversed phase liquid chromatography (LC) and mass spectrometry (LC-MS) users to optimize and confirm correct operation of their equipment.
Catalog Number:
(PI88430)
Supplier:
Invitrogen
Description:
Thermo Scientific Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits.
Catalog Number:
(PI88434)
Supplier:
Invitrogen
Description:
Thermo Scientific Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits.
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the
 is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the
is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
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This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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