OPTEM

Description:   The 3C4 monoclonal antibody reacts with the cell surface glycoprotein mouse CD102 (ICAM-2), a ligand for the leucocyte integrin CD11a/CD18 (LFA-1). CD102 is constitutively expressed on T cells, B cells, and at high levels on vascular endothelial cells. It is also expressed on a variety of leukocyte cell lines. The 3C4 antibody blocks the interaction between ICAM-2 and LFA-1.

Description:   The “classical” MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic α chains noncovalently associated with the invariant β2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny. The monoclonal antibody 28-14-8 binds to the α3 domain of H-2Db in the presence or absence of β2 microglobulin. It cross reacts with the α3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. The antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells.

Description:   Goat Anti-Human IgM-Alexa Fluor 488 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blotting assays.

Description:   Human IgA Lambda-AP for use as a control in ELISA assays.

Description:   PE Anti-Goat IgG Fc antibody for use in fluorescent assays.

Description:   Goat Anti-Mouse IgG2c-FITC for use in fluorescent assays.

Description:   The CD23 antigen is the low affinity IgE Fc receptor, which is a 45-49 kDa protein with 38 and 28 kDa fragments. It is expressed on most mature, conventional B cells (but not on peritoneal CD5+ B cells) and can also be found on the surface of T cells, macrophages and platelets. It is distinct from the high affinity IgE receptors found on basophils and mast cells which mediate allergic reactions. The low affinity receptors are thought to play a role in isotype specific immunoregulation. The regulation of CD23 surface expression appears to be integral with the complex IgE system which involves interactions of cells, cytokines, antibodies and regulatory factors. CD23 has been described as a “membrane bound cytokine," in that the soluble cleavage products of CD23 are themselves able to act as cytokines in vitro.

Description:   Goat Anti-Mouse IgG(H+L)-PE antibody for use in fluorescent assays.

Description:   Goat Anti-Mouse IgM-Alexa Fluor 647 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blotting assays.

Description:   FITC Anti-Mouse IgG3 antibody for use in flow cytometry assays.

Description:   Low Endotoxin/Azide Free Goat Anti-Human IgG antibody for use in ELISA, flow cytometry, immunohistochemistry / immunocytochemistry, western blotting, immunoprecipitation, ELISpot, multiplex, and depletion assays.

Description:   Goat Anti-Mouse Kappa-PE antibody for use in flow cytometry assays.

Description:   Purified Goat Anti-Rat IgG antibody with minimal reactivity to mouse proteins for use in ELISA, flow cytometry, immunohistochemistry / immunocytochemistry, western blotting, and separation assays.

Description:   CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e. B220+/CD43+/HSA+) through fully differentiated B cell stages. Terminally differentiated plasma cells do not express CD19. In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. All splenic and peritoneal IgM+ cells of both B-1 and B-2 lineages are CD19+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites. Studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow.

Description:   ChT1, a member of the Ig superfamily with one V-like and one C2-like domain, is a T cell antigen that is expressed on the surface of embryonic thymocytes (day 10). In young chickens about 90% of the thymocytes as well as a subpopulation of peripheral lymphocytes, which represent recent thymic emigrants are ChT1 positive. Expression in the periphery declines with age and, in correlation with partial thymectomy, indicates that ChT1 can be used as an accurate marker for studying thymic function. The monoclonal antibody CT1 also recognizes the ChT1 antigen in quail cortical thymocytes.

Description:   Goat Anti-Rat IgM-FITC antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blotting assays.